吡喹酮抗体内培养欧猬迭宫绦虫裂头蚴的作用与评价

Effect and evaluation of praziquantel on Sparganum erinaceieuropaei in vivo

目的探讨吡喹酮经灌胃及注射两种方式抗SD大鼠体内欧猬迭宫绦虫裂头蚴的效果。方法将50只SD大鼠每只感染5条欧猬迭宫绦虫裂头蚴,培养两周后随机分组进行药物治疗。灌胃治疗组:使用吡喹酮药物浓度分别为600 mg/kg、1 200 mg/kg、1 800 mg/kg及2 400 mg/kg进行灌胃治疗7 d,灌肠对照组以单纯植物油灌肠;注射治疗组:配制30%吡喹酮注射液以注射剂量分别为0.2 ml和0.5 ml进行注射治疗3 d及6 d,注射对照组以无菌生理盐水注射。疗程结束后剖杀大鼠,计算减虫率,如大鼠体内剩余虫体则进行切片及HE染色。结果吡喹酮作用于大鼠体内裂头蚴,各用药组减虫率与对照组减虫率差异均有统计学意义(P<0.05),其中灌胃法以1 800 mg/kg剂量组减虫率最高,注射法以0.5 ml 6 d组减虫率最高。虫体切片染色后观察,体壁有明显的断裂,甚至深入基质区。结论吡喹酮抗体内裂头蚴虫体有良好的效果,其抗虫机制可能与吡喹酮对裂头蚴组织结构的破坏而致虫体死亡有关。

Objective To explore the effect of praziquantel on Sparganum erinaceieuropaei in SD rats by gas-tric perfusion and injection. Methods Fifty SD rats were selected and infected with five plerocercoids each. After two weeks, the 50 SD rats were randomly divided into 10 groups for drug treatment：perfusion treatment groups with the concentrations of praziquantel of 600 mg/kg, 1 200 mg/kg, 1 800 mg/kg, 2 400 mg/kg, 0 mg/kg （vegetable oil in-stead, the perfusion control group） for 7 d;injection treatment groups with 30%praziquantel of 0.2 ml, 0.5 ml for 3 d and 6 d, normal saline （the injection control group）. After the treatment, rats were sacrificed. The reduction rate of plero-cercoid was calculated. The remaining plerocercoid in rat was sliced and stained by HE. Results The reduction rate showed statistically significant difference in the treatment group and the corresponding control groups （P〈0.05）. The re-duction rate of 1 800 mg/kg group was the highest in perfusion treatment groups. The reduction rate of 0.5 ml for 6 d group was the highest in injection treatment groups. The plerocercoid was stained and the body wall has obvious frac-ture, even deep into matrix area. Conclusion The effect of praziquantel on plerocercoid is significant. The mecha-nism may be related to that praziquantel causes damage in organizational structure of plerocercoid and then thus re-sults in death.