植物细胞有丝分裂实验方法优化

Experimental exploration about optimizing plant cell mitosis scheme

以大蒜根尖为材料，比较不同处理方案下的实验效果，找到植物细胞有丝分裂实验的最佳方案。实验结果显示：观察大蒜根尖有丝分裂最佳培养温度为4℃，分裂指数达40．70％；最佳培养时间为3d，分裂指数达29．00％；10∶00～11∶00为最适取材时间，分裂指数为29．00％．此外，当解离液中浓盐酸与95％乙醇体积比为4∶1、染液为1g／L的番红时染色效果较好，能清晰地显示有丝分裂相。

A variety of Pretreatment regimens were set with the garlic root tip.Comparing the experimental results under dif-ferent treatments were to find the best scheme of the plant cell mitosis experiment.The results show that：the best culture tem-perature for observing the plant cell mitosis is 4℃, with the mitotic index up to 40.70%;the best culture time is 3 days, with the mitotic index up to 29.00%;the most optimal time for sampling is 10∶00-11∶00, with the mitotic index up to 29.00%. In addition, the mitotic characters could be observed clearly when the volume ratio of hydrochloric acid and alcohol in the dis-sociation solution is 4∶1 and the dye is 1g/L of safranin.