刺五加皂苷对高脂高糖饮食大鼠学习记忆能力的影响

Effects of Acanthopanax senticosus saponins on learning and memory abilities of high fat / high sugar diet rats

目的:研究刺五加皂苷对高脂高糖饮食大鼠学习记忆能力的影响和机制。方法:将24只大鼠随机分为对照组、高脂高糖饮食组和刺五加皂苷干预组,每组各8只。通过高脂高糖饮食建立大鼠模型,刺五加皂苷腹腔注射进行干预,Y迷宫实验检测大鼠学习记忆能力,电生理实验检测海马CA1区长时程增强,Westernblot方法检测海马GSK-3β和磷酸化GSK-3β的表达。结果:刺五加皂苷组大鼠Y迷宫学习和记忆成绩明显高于高脂高糖饮食组,刺五加皂苷能增强高脂高糖饮食大鼠海马CA1区长时程(long-term potentiation,LTP),并且抑制海马脑组织中GSK-3β磷酸化。结论:刺五加皂苷能改善高脂高糖饮食大鼠学习记忆能力和降低突触的可塑性,其机制与抑制海马GSK-3β磷酸化有关。

Objective： To observe effects of Acanthopanax senticosus saponins （ASS） on learning and memory abilities of high fat/ high sugar diet （HF/ HS） rats and discuss its mechanism. Methods： 24 male SD rats were randomly divided into control group, HF/ HS group and ASS group with 8 rats in each group. The rats in HF/ HS group and ASS group were fed with high fat/ high sugar diet for 60 days to establish rat model. Then, the rats in ASS group were treated with ASS solution through intraperitoneal injection. After that, the learning and memory abilities were detected with Y maze test. The electrophysiological method was used to observe the long-term potentiation （LTP） in hippocampal CA1 area, and Western blot was used for expression of glycogen synthase kinase-3β （GSK-3β） and phosphorylated GSK-3β （p-GSK-3β） in the hippocampal tissue. Results： The learning and memory abilities of ASS group were significantly better than those of HF/ HS group through the Y maze test. ASS could improve the LTP in hippocampal CA1 area of HF/ HS rats, and inhibit the phosphorylation of GSK-3β in the hippocampus. Conclusions： ASS can improve learning and memory a-bilities and reduce synaptic plasticity of high fat/ high sugar diet rats, and these effects could be associated with the inhibition of phos-phorylation of GSK-3β.