摘要
[目的]观察温阳通窍中药复方含药血清对C6脑胶质瘤细胞增殖的影响。[方法]采用MTT法测定样品对体外培养的肿瘤细胞生长的抑制作用,并在药物代谢不同时间(2 h,4 h,6 h)检测不同剂量的温阳通窍中药复方对C6脑胶质瘤细胞的抑制作用,同时进行空白组对照比较。[结果]温阳通窍中药复方对C6细胞的IC50值约为30.29μg/m L,细胞毒效果欠佳。10%血清浓度下,药物代谢2、4、6 h后,高、低剂量组对C6脑胶质瘤细胞的抑制率分别为30.22%、21.6%、28.3%和32.87%、9.86%、47.98%。[结论]温阳通窍中药复方对C6脑胶质瘤细胞的增殖具有一定抑制作用,但其抑瘤作用不存在时间与剂量依赖关系,这一结果或许与药物在体内代谢、次生活性产物的出现相关。
[Objective] Inspect the impact that secrum has Wenyang Tongqiao prescription on C6 glioma proliferation. [Methods] Utilize MTT to determine the inhibition effect that the sample has to the grow of tumor cell cultivated in vitro, and to determine the inhibition effect that the different dosages of Wenyang Tongqiao prescription of traditiond Chinese medicinef(TCM) have to C6 glioma in different times of drug metabolism(2 h,4 h,6 h),at the same time, comparing the blank control group. [Results] IC50 of Wenyang Tongqiao prescription that impact on the C6 glioma is 30.29 μg/m L approximately and has less cytotoxic effect. Under the 10% concentration secrum,drug metabolism after 2 h,4 h,6 h, the inhibition rates that the high and low dosages to C6 glioma are 30.22%,21.6%,28.3% and32.87%,9.86%,47.98%. [Conclusion] Wenyang Tongqiao prescription has certain inhibitory effect on C6 glioma proliferation, but this effect has no relationship with time and dosages, this result perhaps has a relationship with the drug metabolism and the secondary active products.
出处
《天津中医药》
CAS
2015年第5期282-285,共4页
Tianjin Journal of Traditional Chinese Medicine
基金
天津市科技计划项目(11JCYBJC12500)
天津市中医药管理局
中西医结合科研专项课题(13085)