FK506结合蛋白5基因多态性与重性抑郁障碍的关联研究

The association study between polymorphisms of FK506-binding proteins 5 gene and major depressive disorder

目的 探讨FK506结合蛋白5（FK506-binding proteins 5,FKBP5）基因rs1360780多态性与中国汉族人群重性抑郁障碍（major depressive disorder,MDD）之间的关联.方法 以250例MDD患者及300例健康对照作为研究对象,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对FKBP5基因rs 1360780进行分型,比较研究组和对照组之间基因型和等位基因频率分布差异.并采用t检验、Hardy-Weinberg平衡检验及x2检验进行统计学分析.结果 （1）病例组FKBP5 rs1360780位点基因分布（CC、CT、TT基因型频率分别为59.6％、36.0％、4.4％,C、T等位基因频率分别为78.2％和21.8％）与对照组（CC、CT、TT基因型频率分别为59.0％、36.3％、4.7％,C、T等位基因频率分别为77.2％和22.8％）相比,均差异无统计学意义（P=0.983;P=0.682）;（2）在男性中,病例组FKBP5 rs1360780位点基因分布（CC、CT、TT基因型频率分别为60.3％、33.8％、5.9％,C、T等位基因频率分别为77.2％和22.8％）与对照组（CC、CT、TT基因型频率分别为78.6％、19.0％、2.4％,C、T等位基因频率分别为88.1％和11.9％）相比,均差异有统计学意义（P=0.046;P=0.012）,男性中携带T等位基因的人群患MDD的危险度是携带C等位基因的2.185倍（OR=2.185,95％ CI=1.181～4.042）.（3） FKBP5基因rs 1360780位点多态性可能与MDD患者的HAMD总分,迟滞、睡眠、Maier、焦虑/躯体化以及核心因子分均无显著关联（P＞0.05）.结论 FKBP5基因rs1360780位点多态性可能与中国男性汉族人群MDD发病相关,且携带T可能增加男性患MDD的危险性.

Objective To explore the association between FKS06-binding proteins 5 （FKBP5） gene （rs1360780） polymorphisms and major depressive disorder （MDD） in Han Chinese.Methods Genotypes and allele frequencies of rs1360780 of FKBP5 gene were examined in 250 patients and 300 healthy controls by the method of polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）.T-test,Hardy-Weinberg equilibrium and chi-square test were used to conduct statistical analysis.Results （1） The FKBP5 genotypes and alleles frequency distribution between MDD patients （CC,CT,TT genotypes：59.6%,36.0%,4.4%.C,T alleles：78.2%,21.8%） and controls （CC,CT,TT genotypes：59.0%,36.3%,4.7%.C,T alleles：77.2%,22.8%） had no significant difference （P=0.983.P=0.682）.（2） However,the frequency of FKBP5 genotypes and alleles distribution between MDD patients （CC,CT,TT genotypes：59.6%,36.0%,4.4%.C,T alleles：78.2%,21.8%） and controls （CC,CT,TT genotypes：59.0%,36.3%,4.7%.C,T alleles：77.2%,22.8%） had statistically difference in male subjects （P=0.046.P=0.012） and subjects with T allele have a higher risk compared to those with C allele to develop MDD （OR=2.185,95% CI =1.181-4.042）.（3） The score of HAMD,retard factor,sleep factor,Maier factor,anxiety/somatization factor and core factor in MDD patients with different genotypes had no significant difference （P〉0.05）.Conclusion The results suggest that FKBP5 rs1360780 polymorphisms are associated with the onset of MDD in male Han Chinese.T allele is a risk factor of MDD.