羟基磷灰石涂层表面蛋白质吸附研究及其对细胞黏附和成骨分化的影响

Role of adsorbed proteins on hydroxyapatite-coated titanium in osteoblast adhesion and osteogenic differentiation

通过等离子体喷涂的方法在纯钛表面修饰羟基磷灰石涂层,可有效促进成骨分化并加速骨再生.生物材料表面吸附的蛋白质对后续细胞行为起着至关重要的作用,然而其潜在机理还有待研究和证实.本研究检测了羟基磷灰石涂层（HA-Ti）和纯钛表面（Ti）吸附蛋白质层的性质,研究了蛋白质层性质对2种材料表面细胞黏附状态、细胞活性和成骨分化的影响．

The method of plasma-spray coating of hydroxyapatite(HA) onto pure titanium has been demonstrated to be effective to enhance the osteogenic differentiation and accelerate bone regeneration. Yet it is still a big challenge to figure out the interplay among implant surface properties, adsorbed proteins and cell-surface interactions. In this study, the plasma-sprayed HA-coated titanium(HA–Ti) surface was compared with the titanium substrate in terms of protein adsorption, cell adhesion and differentiation. The phase composition, wettability and topography were characterized. Compared to the Ti substrate,the HA–Ti had a smaller water contact angle, but larger micro-scale roughness, and showed a poorer ability to adsorb fibronectin(Fn), bovine serum albumin(BSA) and serum proteins. However, it could adsorb larger amount of recombinant human bone morphogenetic protein 2(BMP-2). The osteoblasts and bone marrow mesenchymal stem cells(BMSCs) tended to adhere on the Ti substrate. By contrast, the BMSCs cultured on the HA–Ti showed a stronger tendency toward osteogenesis differentiation.