电化学发光法和酶联免疫吸附法对HBSAg和HBSAb测试结果的评价

Electrochemiluminescence immunoassy versus enzyme-linked immunosorbent assay in detection of HBSAg and HBSAb

目的:探讨电化学发光法(ECLIA)和酶联免疫吸附法(ELISA)在乙型肝炎病毒血清标志物检测中的差异。方法:分别使用ECLIA和ELISA法对2013年1-12月广州医学院荔湾医院收集的标本进行HBV-M检测,分析两种检测方法的差异及临床应用价值。结果:两种检测方法测定HBs Ag的阳性率比较,差异无统计学意义(P>0.05),有较好的一致性(Ka Pp=0.96)。ELISA法测定HBs Ab的S/CO比值>11.0者156例,其中152例ECLIA法测定值>100 mlU/mL,一致性达97.4%。结论:ELISA法测定HBs Ag与ECllA法测定结果一致;与ECLIA相比,ELISA测定的HBs Ab具有肯定的免疫保护作用的S/CO比值>11.0;不具有免疫保护作用的S/CO比值<5。

Objective：To investigate the differences between electrochemiluminescence immunoassay （ ECLIA） and enzyme-linked immunosorbent assay （ ELISA） in the detection of hepatitis B virus （ HBV） serum markers.Methods：The serum samples collected in our hospital from January to December in 2013 were tested by ECLIA and ELISA respectively for HBV-M detection in order to analyze the differences and clinical value of the two detection methods.Results：The HBsAg positive rate detected by ELISA and ECLIA did not differ statistically （P〉0.05）,and the results showed a good consistency between both approaches （KaPp=0.96）.The S/CO ratios of HBsAb tested by ELISA of 156 cases were 〉11.0,including 152 cases with〉100 mlU/mL values tested by ECLIA,and the consistency reached 97. 4%. Conclusion：The results of HBsAg detected by ELISA and ECLIA were consistent. Compared with ECLIA, the S/CO ratio of HBsAb detected by ELISA with positive immune protective effect was 〉11.0;and S/CO ratio without immune protective effect was〈5.