微小RNA-223及高迁移率族蛋白-1在脓毒症患儿中的表达及意义

Expression of plasma micro RNA-223 and HMGB-1 in pediatric sepsis patients and its clinical significance

目的观察脓毒症患儿血浆中微小RNA-223(mi R-223)及血清高迁移率族蛋白-1(HMGB-1)的表达。方法选取49例脓毒症患儿,其中一般脓毒症24例、严重脓毒症25例,同时选取50例健康儿童作对照组,检测及比较血浆中mi R-223及血清HMGB-1的表达水平。结果严重脓毒症组、一般脓毒症组及对照组mi R-223、HMGB-1表达的差异有统计学意义(F=63.02、76.32,P<0.05)。mi R-223和HMGB-1预测脓毒症的受试者工作特征曲线(ROC)下面积分别为0.904(95%CI:0.821-0.998),0.748(95%CI:0.625-0.903)。mi R-223与HMGB-1呈正相关(r=3.532,P<0.05)。结论脓毒症患儿外周血中mi R-223及HMGB-1表达水平升高,两者联合检测对早期诊断脓毒症具有一定的临床价值。

Objective To investigate the changes of plasma micro RNA-223（miR-223） and HMGB-1 in pediatric sepsis patients. Methods There were 49 children with sepsis enrolled in the study（sepsis group）,severe sepsis group（n=25） and general group（n=24）. Meanwhile, 50 healthy children（normal control group） were selected as control group. The expression levels of plasma miR-223 and HMGB-1（high mobility group box 1） were detected. The predictive values of miR-223 and HMGB-1 in plasma of children with sepsis were evaluated by receiver operatingcharacteristic（ROC） curve. Results The plasma miR-223 and HMGB-1 expression levels in severe sepsis group and general group were up-regulated compared with those in the normal control group（F=63.02, 76.32, P〈0.05）. The area under ROC curve of miR-223,HMGB-1 predicting sepsis were 0.904（95%CI 0.821-0.998）, 0.748（95%CI： 0.625-0.903）. There was positive correlation between miR-223 and HMGB-1（r=3.532, P〈0.05）. Conclusions The expression levels of plasma miR-223 in children with sepsis are significantly up-regulated, which can be used as early diagnostic markers to reflect the severity of inflammation in some degree.