大网膜脂肪对胆管瘢痕狭窄作用的实验研究

Experimental Study on the Effect of Omental Adipose on Bile Duct Stricture Scar

目的电灼损伤胆管致胆管瘢痕狭窄动物模型,观察大网膜脂肪组织对胆管瘢痕狭窄的作用.方法25只日本大耳兔随机分为A、B、C 3组,胆管电灼损伤处未用大网膜包裹A组、大网膜包裹B组及假手术C组,术后30 d通过观察胆管瘢痕大体形态和HE染色光镜下大网膜脂肪对胆管瘢痕狭窄的作用.结果 A B 2组胆管在大体形态上均可见损伤处形成狭窄,狭窄以上胆管扩张,而C组胆管无改变;A B 2组在HE染色光镜下观察均见胆管壁增厚,胶原纤维明显增生,纤维排列紊乱,其中B组还可见数个脂肪细胞进入致密的胆管瘢痕胶原纤维组织中并被其包绕,且脂肪组织和胆管瘢痕组织能很好的相互融合在一起.结论大网膜脂肪组织对胆管瘢痕狭窄有融合、封堵的作用,为大网膜脂肪组织防治胆管瘢痕狭窄提供了实验依据.

Objective To establish the animal model of bile duct strictures by electrocautery injury, and observe the effect of omental adipose＇ on the Bile duct stricture scar. Methods 25 Japanese rabbits were randomly divided into three groups, electrocautery bile duct lesions without omental wrap （A） , electrocautery bile duct lesions by omental wrap （B） , sham operation （C） were applied respectively. The bile duct stenosis and the omental adipose＇ effect were observed by gross morphology and Stained by HE 30 days after surgery. Results The Bile duct stricture and the bile duct cholangiectasis were observed by gross morphology in the both of A and B group, but there was no change in the sham operation group. The Bile duct fibrosis were observed by HE in the both of A and B group. Adipose cells grew into the collagen fiber organization and were surrounded by dense collagen fibers in B group. Adipose tissue and bile duct scar tissue collagen fibers blended with each other well. Conclusions Omental adipose tissues play a role of fusion and plugging in the bile duct stricture scar. Omental adipose tissues provide a experimental basis for the prevention and treatment of bile duct stricture scar.