载脂蛋白E基因敲除小鼠脑部TNF-α和IL-1β及IL-8的水平升高

Apo E gene knockout causes high expressions of TNF-α,IL-1β and IL-8 in mouse brain

目的探讨载脂蛋白E基因敲除(Apo E-/-)后小鼠脑部海马区和皮质区肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)及IL-8表达变化。方法 4周龄野生型和Apo E-/-雄性小鼠各10只,饲养12周后断颈处死。采集血液样本进行血脂水平检测;将脑组织固定后切片,HE染色观察形态学改变;免疫组织化学染色检测TNF-α、IL-1β、IL-8蛋白表达。结果IL-1β和IL-8在Apo E-/-小鼠海马区和皮质区表达的阳性细胞数均显著高于野生型小鼠。TNF-α在Apo E-/-小鼠皮质区表达的阳性细胞数显著高于野生型小鼠,Apo E-/-小鼠海马区TNF-α的表达强度高于野生型小鼠。结论 Apo E-/-小鼠脑部TNF-α、IL-1β、IL-8表达增加。

Objective To investigate the expression profile of tumor necrosis factor α （TNF-α）, intedeukin 1β （IL-1β） and IL-8 in the hippocampus and cerebral cortex of apolipoprotein E （ApoE） knockout （ApoE-/- ） mice. Methods Twenty 4-week-old male mice were divided into 2 groups： wild-type mice and ApoE-/- mice, 10 mice for each. After 12-week feeding, the blood sample was taken for serum lipid test and brain tissue were obtained for fixation and embedding. The histological changes of the hippocampus and cerebral cordex were observed by HE staining and the expressions of TNF-α, IL-1β and IL-8 proteins were detected by immunohistochemistry. Results Compared with the wild-type mice, the numbers of the IL-115 and IL-8-positive cells were markedly elevated in the hippocampus and cerebral cortex in ApoE-/- mice. The number of the TNF-α-positive cells was markedly raised in the cerebral cortex after ApoE knockout, and the intensity of TNF-α positive substances in the hippocampus is higher in ApoE-/- mice than in wild-type mice. Conclusion The expressions of IL-1β and IL-8 in the brain increased after ApoE knockout in mice.