CO_2气腹压力对胃癌细胞侵袭黏附分子表达的影响

Effect of varying pneumoperitoneum carbon dioxide pressures on adhesive and invasive capacity of gastric cancer cells

目的:探讨不同压强CO2气腹对胃癌细胞侵袭黏附能力的影响。方法:建立体外CO2气腹模型,选胃中分化腺癌株SGC-7901,分别暴露在6、9、12、15 mm Hg(1 mm Hg=0.133 k Pa)和常规细胞培养环境下各3 h。Real-time PCR法检测胃癌细胞中黏附侵袭分子E-cadherin、细胞黏附侵袭分子-1(ICAM-1)、基质金属蛋白酶-2(MMP-2)和细胞黏附分子CD44v6的表达水平;Westernblot检测胃癌细胞中E-cadherin、ICAM-1、MMP-2和CD44v6蛋白的表达水平。结果:随着CO2压强升高,在15 mm Hg时,E-cadherin的mRNA相对表达水平较对照组明显下降(P<0.05);MMP-2和CD44v6的mRNA相对表达水平较对照组明显上升(P<0.05);而ICAM-1 mRNA的相对表达水平也有上调趋势,但各组与对照组比较均无统计学差异。Western-blot检测结果显示蛋白的表达水平与基因检测结果一致。压强为15 mm Hg时,CD44v6和MMP-2的表达上调,E-cadherin的表达下调,具有统计学差异。结论:在压强不高于12 mm Hg时,不同压强的CO2气腹对胃癌细胞株的黏附侵袭能力无明显影响,并不增加肿瘤的转移几率。

AIM： To investigate the effect of va- rying pneumoperitoneum carbon dioxide pressures on adhesive and invasive capacity of gastric cancer cells. METHODS： With an artificial CO2 pneumo- peritoneum model vitalize in vitro, human gastric cancer cell line （SGC-7901） was exposed to CO2 at different pressures for 3 h in each case ：6 mm Hg （ 1 mm Hg =0.133 kPa）,9 mm Hg,12 mm Hg , 15 mm Hg and under conventional cell culture condi- tions. The expressions of mRNA of E-cadherin, ICAM-1, MMP-2 and CD44v6 were measured by Re- al-time PCR. The expressions of protein of E-cadher- in,ICAM-1,MMP-2 and CD44v6 were measured by WB. RESULTS ： With the increase in pressure, RT- PCR showed that there was a downward trend in the expression of E-cadherin mRNA while there were upward trends in the expressions of ICAM-1, MMP-2 and CD44v6 mRNA. Western-blot analysis showed that the expression level of protein was consistent with the result of genetic testing. When the pressure was 15 mm Hg,the expressions of CD44v6, MMP-2 and E-cadherin had significant difference compared with the control group （P 〈 0.05 ）. CONCLU- SION： When the pressure is not higher than 12 mm Hg , the adhesive and invasive capacity of SGC- 7901 are not affected, indicating that pneumoperito- neal CO2 pressure may not increase tumor metasta-sis.