夏枯草总三萜对乙醛刺激的肝星状细胞作用及部分机制

Effects of total triterpenoids of prunella vulgaris L.on acetaldehydestimulated hepatic stellate cell-T6 and partial mechanisms

目的:探讨夏枯草总三萜(TTP)对乙醛刺激的大鼠肝星状细胞(HSC)-T6增殖、凋亡的影响及部分作用机制。方法:用不同浓度的TTP(10、30、100、300、1000μg/m L)对乙醛刺激的HSC-T6进行处理,四甲基偶氮唑蓝(MTT)法检测细胞增殖;流式细胞仪检测细胞凋亡;RT-PCR法检测HSC-T6中平滑肌肌动蛋白(α-SMA)、Ⅰ型前胶原(ProcollagenⅠ)、TGF-β信号转导通路下游中介分子Smad2、Smad3、Smad7 mRNA表达。结果:TTP可抑制乙醛刺激的大鼠HSC-T6增殖,诱导其凋亡,下调HSC-T6中α-SMA、ProcollagenⅠ、Smad2、Smad3的mRNA表达,上调Smad7的mRNA表达。结论:TTP对乙醛刺激的HSC-T6增殖具有抑制作用,并能够促进其凋亡,下调α-SMA、ProcollagenⅠ表达量,其机制可能与TTP能抑制Smad2、Smad3表达,升高Smad7的表达,从而对TGF-β/Smad信号通路发挥调控作用有关。

AIM： To investigate the effects of total triterpenoids of prunella vulgaris L. （TTP） on proliferation and apoptosis in hepatic stellate cell-T6 （HSC-T6） stimulated by acetaldehyde and its par- tial mechanisms. METHODS： HSC-T6 cells were stimulated by acetaldehyde and treated with different doses of TTP （10, 30, 100, 300, 1000 μg/mL）, the HSC-T6 proliferation were detected by MTT, the apoptosis rate were analyzed by flowcytometry, and the expression of mRNA levels of α-SMA, Procolla- gen I , smad2, smad3, smad7 were detected by RT- PCR. RESULTS： As compared with the model group, TTP could inhibit HSC-T6 stimulated by ac-etaldehyde and induce the apoptosis. Furthermore, TI＇P reduced the mRNA expression of α-SMA, Pro- collagen I , Smad2, Smad3 and increase Smad7. CONCLUSION： TIP can inhibit the proliferation of HSC-T6 stimulated by acetaldehyde and induce the apoptosis, down-regulate the expression of α- SMA, Procollagen I, which might be associated with the inhibition of the expression of Smad2, Smad3 and the increase of Smad7.