健脾化湿颗粒对腹泻型肠易激综合征模型大鼠结肠运动及中枢c-fos的影响

Inl uence of Jianpi Huashi granule on colonic motility and c-fos expression in the central nerve system in rats with diarrhea-predominant irritable bowel syndrome

目的:观察健脾化湿颗粒对腹泻型肠易激综合征(diarrhea-predominant irritable bowel syndrome,D-IBS)模型大鼠结肠运动功能的影响,并从中枢c-fos角度探讨其作用机制.方法:采用番泻叶灌胃结合束缚应激法建立D-I B S大鼠模型,应用健脾化湿颗粒进行干预,采用Maclab/4e四导生理记录仪描记大鼠结肠运动曲线,计算结肠运动指数,并用免疫组织化学法检测脊髓腰膨大、前额叶皮质、海马、下丘脑中c-fos的表达,ELISA法检测脊髓腰膨大和海马中c-fos的含量.结果:与正常组相比,D-IBS模型组大鼠结肠运动指数(939.01±91.96 vs 574.78±53.33)明显增加(P<0.01).中枢c-fos的表达(0.25±0.03 v s 0.08±0.01、0.15±0.02 v s 0.06±0.01、0.23±0.02 vs 0.08±0.02、0.16±0.02vs 0.07±0.01)明显升高(P<0.01).c-fos含量(19.68±1.17 v s 11.10±2.59、12.32±1.22vs 5.38±0.88)明显增加(P<0.01).与模型组相比,健脾化湿颗粒中、高剂量组大鼠结肠运动指数(731.91±67.34、695.20±80.58 vs939.01±91.96)明显降低(P<0.01).中枢c-fos的表达(0.14±0.02、0.10±0.01 vs 0.25±0.03;0.11±0.01、0.09±0.02 vs 0.15±0.02;0.16±0.01、0.12±0.01 vs 0.23±0.02;0.12±0.01、0.09±0.01 vs 0.16±0.02)明显降低(P<0.01).c-f o s含量(16.02±1.58、15.62±1.64 vs 19.68±1.17;8.53±2.05、7.28±1.54vs 12.32±1.22)明显降低(P<0.01).结论:健脾化湿颗粒改善D-IBS模型大鼠结肠运动功能的作用机制,可能与其调节中枢c-fos的表达有关.

AIM： To evaluate the effect of Jianpi Huashi granule on colonic motility and c-fos expression in the central nerve system in rats with diarrhea- predominant irritable bowel syndrome （D-IBS）. METHODS： A rat model of D-IBS was established by intragastric administration of Sennae decoction combined with restraint stress. Rats were divided into several groups. Jianpi Huashi granule was given to rats in Jianpi Huashi groups, pinaverium bromide tablets to rats in the positive group and water to the other groups. Maclab/4e 4-channel physiology recorder was used to record colon motion curves and evaluate colonic motility using motility index. Immunohistochemistry assay was used to detect the expression of c-fos in the spinal horn, prefrontal cortex, hippocampus and hypothalamus. ELISA was used to detect the contents of c-fos and 5-hydroxytryptamine in the spinal cord and hippocampus. RESULTS： In the D-IBS model group, the colonic motility index increased significantly compared to the normal group （939.01 ± 91.96 vs 574.78 ± 53.33, P 〈 0.01）. The expression of c-fos in the spinal horn, prefrontal cortex, hippocampus and hypothalamus was significantly increased in the model group compared to the normal group （0.25 ± 0.03 vs 0.08 ± 0.01, 0.15 ± 0.02 vs 0.06 ± 0.01, 0.23 ± 0.02 vs 0.08 ± 0.02, 0.16 ± 0.02 vs 0.07 ± 0.01, P 〈 0.01 for all）. The contents of c-fos in the spinal cord and hippocampus were increased significantly in the model group compared to the normal group （19.68 ± 1.17 vs 11.10 ± 2.59, 12.32 ± 1.22 vs 5.38 ± 0.88, P 〈 0.01 for both）. After treatment, the colonic motility index in the medium- and high-dose Jianpi Huashi granule groups was significantly decreased （731.91 ± 67.34, 695.20 ± 80.58 vs 939.01± 91.96, P 〈 0.01 for both）, the expression of c-fos was significantly reduced （0.14 ± 0.02, 0.10 ± 0.01 vs 0.25 ± 0.03; 0.11 ± 0.01, 0.09 0.02 vs 0.15 ＋ 0.02; 016 ± 0.01, 0.12 ± 0.01 vs 0.23 ± 0.02; 0.12 ± 0.01, 0.09 ± 0.01 vs 0.16 ± 0.02, P 〈 0.01 for all）, and the content of c-fos was also significantly decreased （16.02 ± 1.58, 15.62 ± 1.64 vs 19.68 ± 1.17; 8.53 ± 2.05, 7.28 ± 1.54 vs 12.32 ± 1.22, P 〈 0.01 for all）.CONCLUSION： Jianpi Huashi granule regulates colonic motility in rats with D-IBS possibly by modulating the expression of c-fos in the central nerve system.