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响应面法优化细菌MY07产IAA液体发酵培养基 被引量:8

Application of response surface methodology for optimization of liquid fermentation medium for IAA secretion by MY07
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摘要 [目的]研究氮源、氮源和矿质元素对菌株MY07产吲哚乙酸量的影响。[方法]在单因素筛选和初始浓度确定试验的基础上,利用响应面分析法对细菌MY07产吲哚乙酸的液体发酵培养基进行优化,采用Box-Benhnken设计和响应面法对碳源、氮源和矿质元素水平进行分析。[结果]结果表明,菌株MY07产IAA的液体发酵培养基最佳组合为:0.94%甘露醇,2.35%KNO3,0.12‰Mg SO4·7H2O,0.13‰Ca Cl2·2H2O,在此条件下的验证试验表明,IAA产量可达到28.16μg/ml。[结论]通过响应面法优化菌株培养基后,有利于提高该菌产生IAA的能力。 [ Objective ] The optimization of liquid fermentation medium for producing IAA by MY07 by response surface methodology. [ Methods] The effects of carbon source, nitrogen source and mineral element on IAA secretion were explored in single factor experi- ments. Based on this, the optimum concetrations of them were determined by Box - Benhnken design combined with response surface analy- sis. [ Results] Mannitol and KNO3 were found to be the best carbon source and nitrogen source, respectively. The optimum concetrations (W/V) of Mannitol,KNO3 ,MgSO4 · 7H20 and CaC12 · 2H20 were to be 0. 94% ,2. 35% ,0. 12%o and 0. 13%o,respectively. The IAA se- cretion in optimized medium reached 28. 16 p,g/ml. [ Conclusion ] The component content of culture medium are optimized by the response surface method, which is beneficial to improve the ability of the MY07 to produce IAA.
出处 《生物技术》 CAS CSCD 北大核心 2015年第2期201-204,共4页 Biotechnology
基金 四川省财政创新能力提升工程项目("烟草促生与生防复合菌系的构建及应用研究" No.2013JCYJ-007) 四川省战略性新兴产业关键核心技术研发项目("秸秆快速腐解技术及复合微生物菌剂研发" SC2013510104007)资助
关键词 液体发酵 培养基 IAA 响应面分析法 liquid - state fermentation, culture medium, IAA, response surface methodology
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