甘草悬浮培养细胞对芦荟大黄素的选择性糖基化

Regio-selective glycosylation of aloe-emodin by cell suspension cultures of Glycyrrhiza uralensis

目的研究甘草悬浮培养细胞对芦荟大黄素的生物转化。方法将芦荟大黄素与甘草细胞悬浮培养细胞共孵育7 d后,培养液经由大孔吸附树脂处理,细胞用甲醇提取,合并两部分浸膏后依次采用Sephadex LH-20凝胶柱色谱、正相硅胶柱色谱、反相半制备HPLC等技术进行分离纯化,利用质谱和核磁共振波谱技术进行结构鉴定。结果分离得到了一个芦荟大黄素的选择性糖基化产物——芦荟大黄素-(3-羟甲基)-O-β-D-葡萄糖苷。结论甘草悬浮培养细胞对芦荟大黄素具有位置选择性糖基化能力。

Objective To investigate the biotransformation of aloe-emodin by cell suspension cultures of Glycyrrhiza uralensis. Methods Aloe-emodin was added into cell suspension cultures of Glycyrrhiza uralensis and incubated for another 7 d. To obtain residues, the culture medium was processed by a macroporous adsorbent resin column or the cells was extracted with MeOH, and then these two residues was separated and purified by Sephadex LH-20 column chromatography, silica gel column chromatographynbsp;and reversed-phase semi-preparative HPLC, successively. Through the processes, a metabolite was achieved. The structure of the metabolite was elucidated on the basis of spectroscopic analyses. Results A regio-selectively glycosylated product, aloe-emodin-3-（hydroxymethyl）-O-β-D-glucoside, was isolated from the biotransformation of aloe-emodin by G. uralensis cell suspension cultures. Conclusion The result indicates that the G. uralensis cell suspension cultures possess the regio-selective glycosylated capacity for aloe-emodin.