摘要
目的 探讨锌指结构转录因子1(zinc finger E-box binding homeobox 1,ZEB1)和E钙黏蛋白在膀胱尿路上皮癌组织和正常膀胱黏膜组织中的表达情况、相关性及其与临床病理特征的关系.方法 收集2008年3月至2011年3月86例膀胱尿路上皮癌患者的手术标本.男51例,女35例.年龄36 ~72岁,平均(64.1±10.2)岁.其中>65岁40例,≤65岁46例.均经病理检查证实为膀胱尿路上皮癌,病理分级:G128例,G2~G358例.TNM分期:Ta~T1期34例,T2~T4期52例.肿瘤单发49例,多发37例.初发55例,复发31例.16例正常膀胱黏膜组织取自BPH患者开放手术标本.年龄51 ~75岁,平均(58.6±18.4)岁.采用免疫组化染色方法分别检测ZEB1和E钙黏蛋白在膀胱癌组织及正常膀胱黏膜组织中的表达情况,分析两者在膀胱癌组织中表达的相关性,以及两者表达情况与患者临床病理特征的关系.结果 ZEB1在膀胱癌组织和正常膀胱黏膜组织中表达的阳性率分别为39.5%(34/86)和0(0/16);在高分级(G2 ~ G3)和低分级(G1)膀胱癌组织中表达的阳性率分别为48.3%(28/58)和21.4%(6/28);在T2~T4期和Ta~T1期膀胱癌组织中表达的阳性率分别为51.9% (27/52)和20.6%(7/34),差异均有统计学意义(P<0.05).E钙黏蛋白在膀胱癌组织和正常膀胱黏膜组织中表达的异常率分别为61.6% (53/86)和81.2%(13/16),差异有统计学意义(P<0.01);在高分级(G2 ~ G3)和低分级(G1)膀胱癌组织中表达的异常率分别为53.4%(31/58)和78.6%(22/28);在T2~ T4期和Ta~T1期膀胱癌组织中表达的异常率分别为51.9%(27/52)和76.5%(26/34),差异均有统计学意义(P<0.05).在膀胱癌组织中,ZEB1的阳性表达与E钙黏蛋白的异常表达间呈负相关(r =0.247,P<0.05).结论 ZEB1和E钙黏蛋白在膀胱癌组织中的表达具有负相关性,且两者的表达与膀胱癌的分期、分级密切相关.
Objective To explore the expression,correlation and clinic-pathological characters of zinc finger E-box binding homeobox 1 (ZEB1) and E-cadherin in normal bladder tissues and bladder urothelial carcinoma(BUC).Methods Immunohistochemical staining was performed on paraffin embedded sections to detect the expression of ZEB1 and E-cadherin in 86 cases of BUC,diagnosed by pathology,between March 2008 and March 2011.Among them,51 cases were male and 35 cases were female.The mean age was (64.1 ± 10.2)years,range 36 to 72 years.40 cases were older than 65 years old,and 46 cases were less than 65 years old.The pathological classification included G1 in 28 cases,G2-G3 in 58 cases.The TNM classification showed that Ta-T1 stage in 34 cases,T2-T4 in 52 cases.Single lesion was found in 49 cases and multi lesion was found in 37 cases.55 cases were primary lesion;the other 31 cases were diagnosed as tumor recurrence.Normal bladder mucosa was obtained from 16 patients with BPH.Their age ranged from 51 to 75 years old,mean (58.6 ± 18.4) years old.Similar immunohistochemical staining about ZEB1 and E-cadherin was performed.The expression of ZEB1 and E-cadherin in different tissue and its relationship with the clinic pathological were further analyzed.Results The positive rate of ZEB1 in bladder cancer group (39.5%,34/86) was significantly higher than that in normal bladder mucosa group (0,0/16) (P 〈 0.01).The positive rate of ZEB1 in high grade bladder cancer (G2-G3) was 48.3% (28/58),which was significant higher than that in low grade bladder cancer (G1) (21.4%,6/28) (P 〈0.05);The positive rate of ZEB1 in muscle-invasive bladder cancer (T2-T4) was 51.9% (27/52),which was significant higher than that in non-muscle-invasive cancer (Ta-T1) (20.6%,7/34) (P 〈 0.01).The abnormal rate of E-cadherin expression in bladder cancer (61.6%,53/86) was significantly lower than that in normal bladder mueosa (81.2%,13/16) (P 〈 0.01).The abnormal expression rate of E-cadherin in high grade bladder cancer (G2-G3) (53.4%,31/58) was significantly lower than that in low grade bladder cancer (G1) (78.6%,22/28)(P 〈 0.05);The abnormal rate of E-cadherin expression in invasive bladder cancer (T2-T,4) was 51.9% (27/52),which was lower than that in non-muscle invasive (Ta-T1) cancer (76.5%,26/34) (P 〈 0.05).In bladder cancer,there was a negative correlation between the abnormal expression of E-cadherin and positive expression of ZEB1 (r =0.247,P 〈 0.05).Conclusion The expressions of ZEB1 and E-cadherin in bladder cancer were negatively correlated,which had high correlated with tumor grade and stage.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
2015年第5期337-341,共5页
Chinese Journal of Urology
基金
天津市科委自然基金青年项目(14JCQNJC12700,13JCQNJC11500)
关键词
锌指结构转录因子
E钙黏蛋白
膀胱癌
免疫组织化学
Zinc finger E-box binding homeobox 1
E-cadherin
Bladder cancer
Immunohistochemistry