山羊妊娠期日粮添加酵母硒对后代羔羊睾丸GPxs表达的影响

The Effect of Yeast Selenium Supplementation in Diet of Pregnant Does on Expression of GPxs in Testes of Offsprings

为探明妊娠母山羊日粮中酵母硒水平对其所产后代公羔睾丸GPxs活性、睾丸组织GPx1,GPx3和GPx4 mRNA表达及其GPx4蛋白表达的影响,采用实时定量PCR进行睾丸GPxs mRNA定量分析,并采用免疫荧光技术对GPx4蛋白定量分析。结果显示妊娠母山羊日粮中添加1.0 mg·kg-1硒组(II组)可极显著提高后代公羔睾丸GPxs的活性(P<0.01)。与对照组相比,II组GPx1 mRNA表达量提高了0.84倍,GPx3 mRNA表达量提高了2.6倍,GPx4 mRNA表达量提高5倍,GPx4蛋白表达量显著增加(P<0.01),但4.0 mg·kg-1硒组后代睾丸GPx4蛋白有过表达现象。妊娠母羊日粮添加1.0 mg·kg-1酵母硒(日粮硒含量1.045 mg·kg-1)可明显提高后代公羔睾丸中GPxs基因的表达。本研究不仅丰富了硒营养调控理论,而且对肉羊生产具有指导意义。

This study was conducted to investigate the effect of yeast selenium supplementation in diet of does during pregnancy on expression of GPxs in testis of their offspring male kids. The mRNA relative expression of GPxs was detected by real-time fluorescent quantitative PCR. The expression of GPx4 protein was analyzed by immunofluorescence assay. The supplementation of 1.0 mg ·kg^-1（ II group） as yeast selenium in diet during pregnancy was significantly increased GPxs activities in testis of their offspring（P 〈 0.01 ）. Compare with GPxs mRNA expression levels in testis of their offspring in control group, GPxl mRNA expression levels in II group were increased by 0.84 times. GPx3 mRNA expression levels in II group were increased by 2.6 times, GPx4 mRNA expression levels in II group were increased by 5.0 times. The expression levels of GPx4 protein in testis of their offspring in II group were significantly increased. The levels of GPx4 protein in group with supplementation of 4.0 mg ·kg^-1 as yeast selenium were over-expressed. The supplementation of 1 mg·kg^-1 as yeast selenium in diet of pregnant does could improve GPxs mRNA expression of testis of offspring kids, the selenium concentration in diet of pregnant does is 1. 045 mg·kg^-1. These conclusions not only richened theory of selenium regulation but also provided the direction for goat production.