贵阳地区丙型肝炎患者病毒基因型与血清病毒载量及血液生化细胞学指标相关性研究

Study on correlation of virus gene type in patients of hepatitis c in Guiyang with serum viral load,blood biochemistry and cytological indicators

目的了解贵阳地区慢性丙型肝炎患者基因型分布状况,分析HCV不同基因型患者病毒载量、肝功能及血液学指标的变化。方法应用逆转录-聚合酶链反应(RT-PCR)技术对临床已诊断为丙肝患者进行HCVRNA载量检测,并使用PCR直接测序法进行HCV基因分型,同时检测ALT、AST水平及血液细胞学指标。并分析基因型与HCV-RNA含量、ALT、AST和血液细胞学指标相关性。结果112例基因分型检测结果1a型4例(3.57%),1b型38例(33.93%),3a型20例(17.86%),3b型20例(17.86%),6a型30例(26.79%);各基因型间HCV-RNA载量与ALT、AST水平差异无统计学意义;血液细胞学指标除WBC外,中性粒细胞数百分比、淋巴细胞百分比、PLT和Hb均差异有统计意义(P<0.05)。结论贵阳地区HCV基因分型与病毒载量、患者肝功能不具有显著相关性,但不同基因型间血液学相关指标具有显著差异。

Objective To know tho distribution of genetic types of chronic hepatitis c patients and analyze the viral load, liver function and serological indicators of different HCV genetic types. Method Applied reverse transcription- polymerase chain reaction （RT-PCR） technology to detect the HCV-RNA load of HCV patients （case group）, and use PCR direct sequencing to conduct HCV genetic typing, and meanwhile fully automatic biochemical analyzer to detect ALT and AST level, and fully automatic blood analyzer of 5 classifications to detect cytological indicators of blood. Analyzed the correlation of genetic types with HCV-RNA contents, ALT, AST and blood cytology indica- tors. Result The result of HCV genetic type of 112 cases shows that type la, 4 cases （3.57%）;type lb, 38 cases （33.93%）; type 3a, 20 cases （17.86%）; type 3b, 20 cases （17.86%）; type 6a, 30 eases （26.79%）; conduct one- way analysis of variance of first-time serum virus RNA quantitative result and genetic types from 112 chronic hepati- tis C patients. Genetic type and HCV-RNA ration shows no statistical difference, nor do the HCV genetic types and serum ALT and AST level. In the results of haematological indicators of different genetic types, except WBC com- parative difference which there were no statistical significantly differences （P〉0.05）, other indicators, such as number of neutrophile granulocytes and lymphocytes, PLT and Hb, there were all statistical significantly differences （P〈0.05）. Conclusion HCV genetic types and viral load of Guiyang area, liver function of chronic hepatitis c pa- tients, and hematological examination have important significance to clinical treatment.