石墨烯/血红蛋白复合薄膜的制备、表征及应用

Preparation,characterization and application of graphene/hemoglobin composite film

基于静电吸附作用,将荷正电的血红蛋白（Hb）与荷负电的聚丙烯酸修饰的石墨烯（PAA-g-Gr）层层自组装制备了多层复合薄膜（{PAA-g-Gr/Hb}n）,并将其用于修饰玻碳电极（GCE）,从而实现对过氧化氢（H2O2）的检测。利用傅里叶红外光谱（FT-IR）和紫外-可见吸收光谱（UV-Vis）对复合薄膜制备过程进行了考察;利用场发射扫描电镜（FESEM）考察了复合薄膜的表面形貌;通过交流阻抗技术（EIS）探究了膜材料的电化学性质;利用循环伏安法（CV）以及计时电流法（CA）考察了复合薄膜修饰电极对H2O2的电化学响应性能。结果表明,该方法制备的复合薄膜对H2O2的线性检测范围为1×10-6~1×10-4 mol/L（RSD=0.999）,检测下限为8×10-7 mol/L。

Using positively charged hemoglobin （Hb） and negatively charged polyacrylic acid （PAA） stabilized gra- phene （PAA-g-Gr） as building bloCks,an uniform {PAA-g-Gr/Hb},composite film on the glassy carbon electrode was pre- pared based on layer-by-layer （LBL） self-assembly technology. The composite film forming process were investigated by fourier transform infrared spectroscopy （FT-IR） and ultraviolet-visible absorption spectrum （UV-Vis）. The surface mor- phology of {PAA-g-Gr/Hb}n was observed by field emission scanning electron microscopy （FE-SEM）. The electrocatalytic response of {PAA-g-Gr/Hb}n modified glassy carbon electrode toward hydrogen dioxide （H2 02 ） is investigated by cyclic voltammetry （CV） and ehronoamperometry （CA）. The results showed that the { PAA-g-Gr/Hb}n exhibited a wide linear response range of 1 × 10,6 ~ 1 ×10-4 mol/L with a correlation coefficient of 0. 999 for the detection of H2O2. And the detec- tion limit （S/N= 3） was determined to be 8×10^-7 mol/L.