同轴与相切套管反应器中藻细胞循环周期分析及普通小球藻培养实验

Light/dark cycle analysis and Chlorella vulgaris cultivation in concentric and tangent double tubes

针对同轴（单排曝气孔）和相切（两排曝气孔）套管光生物反应器,利用Chisti数值模型分析了其循环周期特性;并以普通小球藻为研究对象,采用BG11培养基,在3960lux光强和曝气总量10L/min下,研究了不同反应器结构对藻液中细胞密度、藻液pH、溶氧量变化的影响。结果表明：在相同的曝气流量下,同轴套管循环周期为17.8s,较相切套管的短。培养10d后,同轴套管反应器中细胞密度为3.405×106个/mL,比相切套管高出26.1%;藻液pH都波动到9.0附近,相差微小;同轴套管溶解氧浓度在6.5到7.0mg/L间波动,相切套管中的两排曝气使得在相同曝气量下对藻液的扰动弱,溶解氧浓度偏高（6.9~7.3mg/L）。

Based on the concentric（with one row of aeration pores）and tangent（with two rows of aeration pores） double tubes, the light/dark cycles of them were analyzed by Chisti model, and the effects of structures on the cell density, pH and dissolved oxygen concentration were studied through Chlorella vulgaris（FACHB-1227）cultivation under the light intensity of 39601ux and aeration rate of 10L/min. Results showed under the same aeration flow, the cycle time in concentric double tubes was 17. 8s,shorter than that in tangent double tubes. After 10 days cultivation, the cell density in concentric double tubes was 3. 405 ~ 106 cells/mL, 26.1 ~ higher than that in tangent double tubes. The pH had tiny difference and all remains stable around 9.0, and the dissolved oxygen cormentrfition in the concentric double tubes fluctuated between 6.5 and 7.0mg/L,while the two row of aeration pores in tangent double tubes made lower disturbance,resulting in higher con- centration（6.9 to 7.3mg/L）.