视网膜母细胞瘤蛋白、基质金属蛋白酶3和人细胞周期素D2在上皮性卵巢癌中的表达及临床意义

Expressions of Cyclin-D2, matrix metalloproteinase 3 and retinoblastoma protein in epithelial ovarian cancer and their clinical significance

目的探讨人细胞周期素D2(Cyclin-D2)、基质金属蛋白酶3(MMP-3)和视网膜母细胞瘤蛋白(p Rb)在上皮性卵巢癌癌组织中的表达及与上皮性卵巢癌生物学行为的关系。方法应用免疫组织化学SP法检测80例上皮性卵巢癌和30例正常卵巢组织中Cyclin-D2、MMP-3和p Rb蛋白的表达。结果 Cyclin-D2、MMP-3和p Rb在上皮性卵巢癌癌中的阳性率分别为76.25%、73.75%和41.25%;Cyclin-D2和p Rb蛋白在上皮性卵巢癌癌中的表达与病理类型有关(P<0.05),MMP-3在上皮性卵巢癌中的表达与病理类型无关(P>0.05);Cyclin-D2、MMP-3和p Rb蛋白在上皮性卵巢癌复发组中的阳性率与无复发组比较,差异有统计学意义(P<0.05);Cyclin-D2与p Rb在上皮性卵巢癌中的表达呈负相关,与MMP-3表达呈正相关。结论 Cyclin-D2、MMP-3和p Rb蛋白的异常表达在上皮性卵巢癌发生、发展和复发中起重要作用,联合检测多个指标可能对患者预后的判断有指导意义。

[Objective] To study on expressions of Cyelin-D2,matrix metalloproteinase 3 （MMP-3） and retinoblastoma protein （pRB） in epithelial ovarian cancer and their clinical significance. [Methods] The expressions of Cycfin-D2, MMP-3 and pRB were compared by immunohistochemistry in 80 epithelial ovarian cancer and 30 normal ovarian tissue samples. Correlations of Cyclin-D2, MMP-3 and pRB expressions with clinicopathologieal parameters and post-operative survival were analyzed. [Results] The positive rates of Cyelin-D2, MMP-3 and pRB were 76.25%, 73.75% and 41.25%, respectively. The expressions of Cyclin-D2 and pRB were significantly associated with pathologic classification （P 〈 0.05）, while MMP-3 had no correlation with pathologic classification （P 〉 0.05）. The expression rates of Cyclin-D2, MMP-3 and pRB showed significant differences between the recurrence group and non-recurrence group （P〈 0.05）. There was a significant negative correlation between Cyclin-D2 and pRB protein expressions. There was a significant positive correlation between Cyclin-D2 and MMP-3 expressions. [Conclusion] The abnormal expressions of Cyclin-D2, MMP-3 and pRB have important roles in the development and recurrence of epithelial ovarian cancer. The combined detection of multiple biomarkers may be useful for predicting the prognosis of the patients.