疟疾诊断参比实验室:疟原虫显微镜检测能力外部评估结果分析

Malaria diagnosis reference laboratory:analysis of malaria detecting ability under microscope in the External Quality Assurance Program

目的 评价国家疟疾诊断参比实验室的疟原虫显微镜检测水平。 方法 综合分析该实验室参与2013—2014年世界卫生组织西太区组织的疟原虫血涂片显微镜检测外部质量评估结果。 结果 疟原虫虫种鉴定水平在2013年和2014年的得分率分别为91.11%（82/90）和93.33%（84/90）。但间日疟原虫与卵形疟原虫之间误判率较高。2014年原虫计数的成绩（得分率：93.94%，31/33）好于2013年（得分率：60.61%，20/33）。 结论 该实验室疟原虫镜检能力不断提高，但仍需加强卵形疟原虫的镜检能力，同时提高原虫计数能力。

Objective To evaluate the level of malaria detecting ability under microscope in the national malaria diagnosis reference laboratory. Methods The results of malaria detection under microscope in the External Quality Assurance Program were analyzed. The program was organized by Western Pacific Region Office, World Health Organization in the year of 2013 and 2014. Results The performance of identification of Plasmodium species was good generally （the scoring ratio was 91.11% and 93.33% in 2013 and 2014） ex- cept the poor classification between Plasmodium vivax and Plasmodium ovale. And the quantification of parasite counts in 2014 was significantly better than （scoring ratio： 93.94%, 31/33） that in 2013 （scoring ratio： 60.61%, 20/33）. Conclusion The malaria detecting ability under microscope of national malaria diagnosis reference laboratory was continuously improving, but it is still essential to advance the training on the classifi- cation of rare Plasmodium species under microscope, as well as the parasite counting.