程序性细胞死亡因子4与细胞色素C在上皮性卵巢癌中的表达及其关系

Expression of programmed cell PDCD4 and Cyt C in epithelial ovarian cancer and its relationship

目的：研究上皮性卵巢癌组织中程序性细胞死亡因子4（PDCD4）和细胞色素C（Cyt C）的表达及其关系，探讨PDCD4诱导细胞凋亡的途径。方法收集2010年1月~2015年1月菏泽市立医院妇科62例手术切除的卵巢癌标本，采用免疫组织化学法检测上皮性卵巢癌石蜡标本中PDCD4和Cyt C表达情况，采用配对字2检验分析PDCD4与Cyt C表达的关系。结果62例患者中33例（53.2%）患者PDCD4呈阴性表达，29例（46.8%）患者PDCD4呈阳性表达；40例（64.5%）患者Cyt C呈阴性表达，22例（35.5%）Cyt C呈阳性细胞表达。 PDCD4蛋白表达呈阳性的患者Cyt C表达亦多呈阳性；Cyt C蛋白表达呈阴性的患者PDCD4表达亦多呈阴性，配对χ2检验结果显示，PDCD4和Cyt C的表达具有一定的相关性（χ2=5.652，P〈0.05）。结论上皮性卵巢癌中PDCD4和Cyt C表达密切相关，PDCD4可能通过线粒体途径引起Cyt C释放，启动凋亡程序，诱导细胞凋亡。

Objective To research expression of programmed cell programmed cell death 4 （PDCD4） and Cytc in ep-ithelial ovarian cancer in epithelial ovarian cancer and its relationship, to investigate the pathways of PDCD4 induce apoptosis of pancreatic cancer cells. Methods 62 patients who received pancreatic resection from January 2010 to Jan-uary 2015 in Heze Municiple Hospital were collected. The expression of Cyt C and PDCD4 in the samples was detected by immunohistochemistry, and the relationship between PDCD4 and Cyt C in pancreatic cancer was analyzed by apply-ing paired χ2 test. Results Compared with the regular pancreatic tissues, the expression of Cyt C and PDCD4 in the cancer cells were significantly decreased. Among 62 samples, 37 cases （59.7%） had negative expression of PDCD4, 25 cases （40.3%） had positive expression of PDCD4; 45 cases （72.6%） had negative expression of Cyt C, 17 cases （27.4%） had positive expression of Cyt C. In general, patients who had positive expression of PDCD4 would have negative ex-pression of Cyt C, and the patients who had negative expression of Cyt C would have negative expression of PDCD 4 as well. Paired χ2 test showed that, the expression of those two components were closely correlated （χ2=5.652, P〈 0.05）. Conclusion There is a close relationship between expression of PDCD 4 and Cyt C in pancreatic cancer. PDCD 4 may induce the apoptosis of pancreatic cancer cells through mitochondria.