高效液相色谱-蒸发光散射检测器法测定中药茺蔚子中盐酸水苏碱的含量

Content determination of the hydrochloric acid stachydrine in Mother-wort Fruit by HPLC-ELSD

目的：建立中药茺蔚子中盐酸水苏碱的高效液相色谱-蒸发光散射检测器测定方法。方法 Asahipak NH2P 504E色谱柱（4.6 mm×250 mm），流动相为乙腈-0.2%磷酸溶液（84：16），体积流量1 mL/min，柱温30℃。结果盐酸水苏碱在0.487~4.383μg范围内线性关系良好，r=0.9999，平均回收率为97.96%，RSD为0.92%。结论该法灵敏度高，专属性强，可作为中药茺蔚子中盐酸水苏碱的定量测定方法。

Objective To establish HPLC-ELSD determination method of the hydrochloric acid stachydrine in Mother-wort Fruit. Methods The separation by HPLC-ELSD was performed on Asahipak NH2P 50 4E column （4.6 mm×250 mm） with acetonitrile-0.2% phosphoric acid solution （84：16） as mobile phase, 1 mL/min of flow rate and 30℃ of column temperature. Results Hydrochloric acid stachydrine at 0.487-4.383μg had good linear relationship, r=0.9999, the av-erage recovery was 97.96%, RSD was 0.92%. Conclusion The method is sensitive, specific, it can be used as quantita-tive determination method of the hydrochloric acid stachydrine in Motherwort Fruit.