榼藤子药材质量标准研究

Quality standard study on Entadae Semen

目的：建立榼藤子药材的质量标准,为该药材质量控制提供依据。方法：以榼藤子中主要活性成分榼藤子苷、榼藤子酰胺A-O-β-D-吡喃葡萄糖苷为指标,分别采用TLC和HPLC进行定性鉴别和含量测定研究;参照《中国药典》2010年版附录相关方法,对榼藤子药材的水分、灰分及醇溶性浸出物进行测定。结果：TLC鉴别分离良好,斑点清晰。HPLC含量测定方法学结果表明榼藤子苷在0.014~2.747 g·L-1具有良好的线性关系,r=0.999 6（n=9）,平均回收率为101.06%,RSD 0.90%（n=6）;榼藤子酰胺A-O-β-D-吡喃葡萄糖苷在0.002~0.452 g·L-1具有良好的线性关系,r=0.999 7（n=9）,平均回收率为101.52%,RSD 1.09%（n=6）;21批样品含榼藤子苷质量分数为5.12%~9.24%,榼藤子酰胺A-β-D-吡喃葡萄糖苷的质量分数为0.55%~2.17%。醇溶性浸出物为30.9%~45.2%,水分为6.6%~8.6%,总灰分为2.4%~2.9%。结论：建立的方法可用于榼藤子药材的质量控制。

Objective： To establish the quality standard of Entadae Semen, and provide scientific basis for its quality control. Method： TIE and HPIE were used for qualitative identification and quantitative analysis of phaseoloidin and entadamide A-O-β-D-glucopyranoside in Entadae Semen. The test of water content, ash and ethanol-soluble extractives of Entadae Semen was carried out according to the methods re- corded in appendix of Chinese Pharmacopeia（2010 edition）. Result： The TLC was well separated with clear spots. The linear range of phase- oloidin was between 0. 014 to 2. 747 g · L^-1 （r =0. 999 6, n =9） withan average recovery rate of 101.06% （RSD 0. 90%, n =6） ; the linear range of entadamide A-O-β-D-glucopyranoside was between O. 1302 to 0. 452 g · L^-1 （ r = 0. 999 7, n = 9） withan average recovery rate of 101.52% （RSD 1. 09%, n =6）. The content of phaseeloidin in sample is between 5. 12% to 9. 24%, entadamide A-O-β-D-glucopyranoside is between 0. 55% to 2. 17%, alcohol-soluble extracts is between 30. 9% to 45. 2%, water is between 6. 6% to 8. 6%, and total ash is between 2. 4% to 2. 9%. Conclusion：The established standard is acceptable for quality control of Entadae Semen.