IL-26在胃癌中表达及其生物学机制

Expression and biological mechanism of IL-26 in human gastric cancer

目的探讨白细胞介素26(IL-26)在人胃癌中的表达及其生物学机制。方法采用实时荧光定量PCR和免疫组织化学检测60例胃癌组织及相应癌旁组织中IL-26及其受体IL-20R1的表达。采用IL-26 1、10、50ng/ml刺激人胃癌细胞株MKN45后,采用MTT法和流式细胞术分别检测细胞增殖和凋亡能力,Western blot检测转录激活子3(STAT3)信号通路激活情况。结果胃癌组织中IL-26及其受体IL-20R1表达水平高于癌旁组织(P<0.01)。IL-26呈浓度依赖性地促进MKN45细胞增殖及抗凋亡能力,且上调STAT3磷酸化水平、B细胞淋巴瘤-白血病2(Bcl-2)和细胞周期蛋白D1(cyclinD1)蛋白表达(P<0.05)。结论 IL-26在人胃癌组织中高表达,并且通过激活STAT3信号通路,从而促进胃癌细胞的增殖。

Objective To investigate the expression and biological mechanism of interleukin-26 （IL-26） in human gastric cancer. Methods The expressions of IL-26 and IL-20R1 were detected by RFQ-PCR and immunohistochemistry in 60 cases of gastric cancer and tumor-adjacent normal tissues. Human gastric cancer cell line MKN45 was stimulated with IL-26 1,10 and 50 ng/ml, and then cell proliferation and apoptosis were measured by MTT and flowcytometry, respectively. The activation of STAT3 signaling pathway was determined by Western blot. Results The expressions of IL-26 and II;20R1 in gastric cancer tissues were higher than those in tumor-adiacent normal tissues（P〈0.01）. IL-26 significantly promoted the proliferation and anti-apoptosis of MKN45 cells in a dose-dependent manner, and upregulated the expressions of STAT3 phosphorylation, Bcl-2 and cyclin D1 （P〈0. 05）. Conclusion IL-26 is highly expressed in human gastric cancer and can enhance the proliferation of MKN45 cells via the activation of STAT3 signaling pathway.