结直肠癌患者血浆中DCLK1的表达及意义

The expression of plasmic DCLK1 in patients with colorectal cancer and its clinical signii cance

目的 :检测双肾上腺素样激酶1(doublecortin-like kinase 1,DCLK1)在结直肠癌患者血浆中的表达水平,并通过与癌胚抗原(carcinoembryonic antigen,CEA)的对比,探讨其诊断价值。方法 :收集78例结直肠癌患者手术前的血浆标本和48例健康志愿者的血浆标本,应用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测DCLK1和CEA的表达水平,对比分析DCLK1、CEA及二者联合检测诊断结直肠癌的感敏度、特异度和准确性,并分析血浆中DCLK1和CEA的表达水平与结直肠癌患者临床病理特征之间的关系。结果 :DCLK1和CEA在结直肠癌患者血浆中的表达水平均明显高于健康志愿者(P<0.01)。DCLK1诊断结直肠癌的敏感度和准确性均优于CEA,DCLK1与CEA联合检测可进一步提高诊断结直肠癌的敏感度和准确性。血浆中DCLK1的含量与结直肠癌患者的TNM分期、分化程度、有无淋巴结转移及有无脉管癌栓等相关(P<0.01)。结论 :血浆DCLK1有望成为诊断结直肠癌的一种新型肿瘤标志物,且与结直肠癌患者的恶性程度密切相关。

Objective：To examine the plasmic expression level of doublecortinlike kinase 1（DCLK1） in patients with colorectal cancer and explore its diagnostic value through comparing with carcinoembryonic antigen（CEA）.Methods：The plasma samples were obtained from 78 patients with colorectal cancer before surgical operation and 48 healthy volunteers.The plasmic expression levels of DCLK1 and CEA were examined by enzyme-linked immunosorbent assay（ELISA）.The sensitivity,specificity and accuracy of DCLKl and CEA alone as well as in combination for diagnosis of colorectal cancer were compared.The relationships of the plasmic expression levels of DCLKl and CEA with the clinicopathological features were analyzed.Results：The plasmic expression levels of DCLKl and CEA in patients with colorectal cancer were both higher than those in the healthy volunteers（both P 0.01）.The sensitivity and accuracy of DCLKl for diagnosis of colorectal cancer were both higher than those of CEA.The combination of DCLKl and CEA could further improve the sensitivity and accuracy of diagnosis.The plasmic expression level of DCLKl had a significant impact on TNM staging,degree of differentiation,lymph node metastasis and vascular invasion（all P 0.01）.Conclusion：Plasmic DCLKl is expected to become a novel tumor biomarker for diagnosis of colorectal cancer,and it is associated with the grade of malignancy.