期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

MicroRNA-203对人下咽癌细胞转移能力的影响及其机制研究 被引量：1

Research on functions and mechanisms of Micro RNA-203 on migration of human hypopharyngeal carcinoma cells
下载PDF
导出
摘要 目的研究Micro RNA-203对人下咽癌细胞迁移能力的影响及作用机理。方法利用生物信息学方法预测Micro RNA-203的下游调控靶分子,利用荧光素酶报告基因实验、q RT-PCR、与Westenr blot等方法对其中与调节细胞迁移有关的靶分子MEKK1进行验证,证实下咽癌细胞中MEKK1的表达是否受Micro RNA-203的影响。通过细胞迁移实验研究Micro RNA-203对下咽癌细胞迁移能力的影响,进一步阐明可能影响下咽癌转移能力的因素。结果在过表达Micro RNA-203的下咽癌细胞中,Westenr blot检测靶基因蛋白表达量分析、q RT-PCR检测靶基因m RNA表达量和双荧光素酶报告基因实验结果均提示Micro RNA-203下调靶基因MEKK1的表达。细胞迁移实验证明过表达Micro RNA-203可显著抑制下咽癌细胞的迁移能力。结论 MEKK1是Micro RNA-203的直接下游作用靶点,Micro RNA-203有可能通过负向调控MEKK1抑制下咽癌细胞的转移能力。 Objective To determine the roles and underlying molecular mechanism of MicroRNA-203 on the migration of human hypo-pharyngeal carcinoma cells. Methods The potential MicroRNA-203 target genes were searched by bioinformatic miRNA target prediction tools and KEGG database,and a large number of candidates was identified. The MEKK1 was selected for further investigation. This gene is known to play a role in tumor metastasis. The MicroRNA-203’s binding sites in MEKK1’s mRNA 3’UTR were analyzed by luciferase report-er assays. Nextly,the protein expression of MEKK1 in Fadu-Lv-MicroRNA-203 cells was determined by Western blot assay. The regulation of MEKK1’s mRNA expression by MicroRNA-203 was analyzed by qRT-PCR. Transwell cell migration assays were performed to confirm the im-pact of MicroRNA-203 on hypopharyngeal carcinoma metastasis. Results The expression level of endogenous MicroRNA-203 was negatively correlated with the mRNA and protein expression levels of MEKK1 in hypopharyngeal carcinoma cells. Transwell migration assay results showed that MicroRNA-203 overexpression inhibited hypopharyngeal carcinoma cell migration ability. Furtherly,MEKK1 can promote hypo-pharyngeal carcinoma cell migration ability. Conclusion MEKK1 is a direct target of MicroRNA-203. MicroRNA-203 plays a role in hypo-pharyngeal carcinoma cell migration ability through MEKK1.
作者 高丽 卞卡
机构地区 第四军医大学学员一旅 第四军医大学唐都医院耳鼻咽喉科
出处 《局解手术学杂志》 2015年第3期241-244,共4页 Journal of Regional Anatomy and Operative Surgery
基金 国家自然科学基金青年基金(81202138)
关键词 MicroRNA-203 MEKK1 下咽癌 转移 MicroRNA-203 MEKK1 hypopharyngeal carcinoma migration
分类号 R739.91 [医药卫生—肿瘤]
  • 相关文献

参考文献15

  • 1Jemal A,Siegel R,Xu J,et al.Cancer statistics,2010[J].CA Cancer J Clin,2010,60(5):277-300.
  • 2Leemans CR,Braakhuis BJ,Brakenhoff RH.The molecular biology of head and neck cancer[J].Nat Rev Cancer,2011,11(1):9-22.
  • 3Bian K,Fan J,Zhang X,et al.Micro RNA-203 leads to G1 phase cell cycle arrest in laryngeal carcinoma cells by directly targeting survi vin[J].FEBS Lett,2012,586(6):804-809.
  • 4Wery M,Kwapisz M,Morillon A.Micro RNA-203 leads to G1 phase cell cycle arrest in laryngeal carcinoma cells by directly targeting surv ivin[J].Wiley Interdiscip Rev Syst Biol Med,2011,3(6):728-738.
  • 5Iorio MV,Visone R,Di Leva G,et al.Micro RNA signatures in human ovarian cancer[J].Cancer Res,2007,67(18):8699-8707.
  • 6Ikenaga N,Ohuchida K,Mizumoto K,et al.Micro RNA-203 expression as a new prognostic marker of pancreatic adenocarcinoma[J].Ann Surg Oncol,2010,17(12):3120-3128.
  • 7Furuta M,Kozaki KI,Tanaka S,et al.mi R-124 and mi R-203 are epigenetically silenced tumor-suppressive micro RNAs in hepatocellular carcinoma[J].Carcinogenesis,2010,31(5):766-776.
  • 8Yuan Y,Zeng ZY,Liu XH,et al.Micro RNA-203 inhibits cell proliferation by repressingΔNp63 expression in human esophageal squamous cell carcinoma[J].BMC Cancer,2011,11:57.doi:10.1186/1471-2407-11-57.
  • 9Bueno MJ,Pérez de Castro I,Gómez de Cedrón M,et al.Genetic and epigenetic silencing of micro RNA-203 enhances ABL1 and BCR-ABL1 oncogene expression[J].Cancer Cell,2008,13(6):496-506.
  • 10Li J,Chen Y,Zhao J,et al.mi R-203 reverses chemoresistance in p53-mutated colon cancer cells through downregulation of Akt2 express ion[J].Cancer Lett,2011,304(1):52-59.

二级参考文献51

  • 1邵义祥,吴宝金,薛整风,陈兵,茅慧华,赵明,李厚达.遗传性角膜基质变性小鼠及其突变基因的定位[J].南京师大学报（自然科学版）,2006,29(2):99-102. 被引量：25
  • 2Vuong H, Patterson T, Shapiro P, et al. Phorbol ester-induced expression of airway squamous cell differentiation marker, SPRR1B, is regulated by protein kinase Cdelta/ Ras/MEKK1/MKKl-dependent/AP-1 signal transduction pathway. J Biol Chem, 2000, 275(41 ) : 32250 -32259.
  • 3Xia Y, Makris C, Su B, et al. MEK kinase 1 is critically required for c-Jun N-terminal kinase activation by proinfiammatory stimuli and growth factor-induced cell migration. Proc Natl Acad Sci, 2000, 97 (10): 5243 - 5248.
  • 4Fanger G R, Johnson N L, Johnson G L. MEK kinases are regulated by EGF and selectively interact with Rac/ Cdc42. EMBO J, 1997, 16(16) : 4961 -4972.
  • 5Cuevas B D, Abell A N, Witowsky J A, et al. MEKK1 regulates calpain-dependent proteolysis of focal adhesion proteins for rear-end detachment of migrating fibroblasts. EMBO J, 2003, 22(13): 3346-3355.
  • 6Gallagher E D, Gutowski S, Sternweis P C, et al. RhoA binds to the amino terminus of MEKK1 and regulates its kinase activity. J Biol Chem, 2004, 279 (3) : 1872 - 1877.
  • 7Yan M, Dai T, Deak J C, et al. Activation of stress- activated protein kinase by MEKK1 phosphorylation of its activator SEK1. Nature, 1994, 372 (6508) : 798 - 800.
  • 8Lin A, Minden A, Martinetto H, et al. Identification of a dual specificity kinase that activates the Jun kinases and p38-Mpk2. Science, 1995, 268(5208) : 286 -290.
  • 9Harris M J, Juriloff D M. Eyelid development and fusion induced by cortisone treatment in mutant, lidgap-Miller, fetal mice. A scanning electron microscope study. Embryol Exp Morphol, 1986, 91 : 1 - 18.
  • 10Russell L B. lg-lid gap. Mouse News Lett, 1961, 25 : 64.

共引文献7

同被引文献3

引证文献1

二级引证文献4

局解手术学杂志
2015年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部