短暂饥饿诱导大鼠脑葡萄糖代谢分布改变的研究

Temporary starvation induces changes in glucose metabolism in the rat brain:an ^(18)F-FDG-PET study

目的探究短暂饥饿诱导的大鼠不同脑区葡萄糖代谢的改变。方法将13只雄性Wistar大鼠随机分成两组:对照组(n=6)饮食供应充足,饥饿组(n=7)实验前禁食15 h。大鼠经尾静脉注射18F-FDG后放置到小动物PET-CT仪上扫描。将获取的PET-CT影像学资料与T2WI大鼠脑MRI模板配准。采用Image-J软件手工绘制感兴趣区(ROI),分析大鼠脑内24个区域的18F-FDG摄取的情况。结果与对照组相比,饥饿组大鼠的尾壳核(18.19%)、内囊(18.41%)、丘脑(10.00%)、下丘脑(17.45%)、黑质(15.28%)、腹侧被盖(15.08%)以及脑桥(13.33%)区域18F-FDG摄取升高(P<0.05),而在运动皮层(15.34%)和内嗅皮质(7.04%)区域18F-FDG摄取下降(P<0.05),差异有统计学意义。结论短暂饥饿的大鼠的脑内葡糖糖代谢分布发生了实质性的改变。18F-FDG PET可以反映短暂饥饿条件下不同脑区的激活和抑制。

Objective To explore temporary starvation induced changes in brain glucose metabolism in rats.Methods Thirteen male Wistar rats were used in the present study,and were divided into control（n=6） and starvation groups（n=7）.The rats in control group were supplied with sufficient food and water.While in the starvation group,animals were deprived of food but allowed free access to water for 15 h before the PET-CT scan.Anesthetized rats were injected with18F-FDG via the tail veins.Then rats were scanned for PET-CT,and image data was co-registered with a T2 WI MRI template using SPM8 software.Image J was employed to draw regions of interest（ROIs） from the MRI template and acquire ROI activity information from the PET images.Results In the starvation group vs.controls,18F-FDG uptake（FU） was increased in the caudate putamen（18.19%）,internal capsula（18.41%）,thalamus（10.00%）,hypothalamus（17.45%）,substantia nigra（15.28%）,ventral tegmentum（15.08%） and pons（13.33%,all：P 〈0.05）.However,FU was decreased in motor（15.34%） and entorhinal cortices（7.04%,all：P〈 0.05）.Conclusion The glucose metabolism distribution map in the temporary starvation rat brains were substantially changed versus controls.PET with18F-FDG can demonstrate excitement and inhibition of different brain areas even in cases of temporary starvation.