电针夹脊穴结合跑台训练对兔坐骨神经损伤后胫骨骨质量的影响

Effects of electro-acupuncture on Jiaji acupoints combined with treadmill training on tibia bone mass of rabbit with sciatic nerve injury

目的观察电针夹脊穴及跑台训练对兔坐骨神经损伤后胫骨骨质量的影响，为周围神经损伤后维持正常骨代谢选择更合理的治疗方案，提供理论依据。方法采用随机数字表法将24只新西兰家兔随机分成模型组、假手术组、跑台组和电针跑台组，每组6只。采用钳夹方法对模型组、跑台组、电针跑台组的家兔进行坐骨神经卡压造模，假手术组只将组织切开，不卡压神经。跑台组于造模成功3d后即开始电动跑台康复训练，电针跑台组在跑台组训练方案的基础上增加电针夹脊穴治疗。假手术组和模型组不做任何干预，仅正常饲养。于治疗前（造模后即刻）对4组家兔进行行为学观测，并于治疗4周后（跑台组和电针跑台组治疗4周后）再次对4组大鼠进行行为学观测，同时测定其骨密度和骨强度。结果治疗4周后，模型组、跑台组和电针跑台组的骨密度和骨强度均低于假手术组，差异均有统计学意义（P〈0．05）；电针跑台组的骨密度和骨强度分别为（0．17±0．01）g／cm^2。和（161．92±43．27）N，均显著高于模型组和跑台组，差异有统计学意义（P〈0．05）。结论电针夹脊穴结合跑台训练可显著改善坐骨神经损伤后胫骨的骨密度和强度，促进骨的正常代谢以及维持骨组织的正常功能。

Objective To observe the effects of electro-acupuncture on Jiaji acupoints combine with tread- mill training on tibia bone mass of rabbits with sciatic nerve injury, so as to provide the theoretical support for better project to maintain normal metabolism of bone after peripheral nerve injury. Methods A total of 24 New Zealand rabbits were randomly divided into four groups （n = 6） , namely a model group, a sham group, a treadmill group and an electro-acupuncture ＋ treadmill group according to a random number table. The sciatic nerve of rabbits in the model, treadmill and electro-acupuncture ＋ treadmill group was clamped using hemostatic forceps to establish the model of sciatic nerve injury, while that of the sham group was not clamped, just cut soft tissue around the sciatic nerve. Three days after modeling, the rabbits in treadmill group and the electro-acupuncture ＋ treadmill group were given treadmill training and electro-acupuncture combine with treadmill training respectively, lasting 4 months, whereas those in the sham and the model groups were not given any intervention. Right after modeling, the behavior of rabbits in four groups was observed. Four weeks after treatment or feeding, their bone mineral density and bone strength of tibia were measured. Results Four weeks after treatment, there was significant reduction in bone den- sity and bone strength of tibia in the model, the treadmill and the electro-acupuncture ＋ treadmill groups compared with those in the sham group（P 〈0.05） ; the bone mineral density and bone strength of tibia in the electro-acupunc- ture ＋ treadmill group were 0.17± 0.01 g/cm^2 and 161.92± 43.27 N respectively,significantly higher than those in both the model and the treadmill groups （ P 〈 0.05 ）. Conclusion Electro-acupuncture combined with treadmill training can improve tibia bone mineral density and bone strength after sciatic nerve injury and facilitate the normal metabolism of the bone and to maintain its normal function.