多囊卵巢综合征慢性炎性相关因子及趋化因子生长调节致癌基因-α表达的研究

Growth-regulated Oncogene-α and Other Inflammatory Chemokines Expression in Follicular Fluid Cells in Patients with Polycystic Ovary Syndrome

目的比较各细胞因子和趋化因子在多囊卵巢综合征(PCOS)患者和正常妇女卵巢黄素化颗粒细胞的表达及血清、卵泡液中的水平,初步探讨PCOS慢性炎症的可能机制。方法 2014年1月-8月选择体外受精-胚胎移植中PCOS患者及非PCOS患者各40例,收集其颗粒细胞、卵泡液和血清,分别用实时荧光定量-聚合酶链式反应、酶联免疫吸附法进行检测,观察肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-8及生长调节致癌基因-α(GRO-α)表达的差异,并按是否肥胖(体质量指数>25 kg/m2)分层进行组间比较。结果在肥胖者中,TNF-α、IL-6、IL-8、GRO-α在PCOS组的表达高于对照组,差异有统计学意义(P<0.05);在非肥胖者中,TNF-α、IL-8、GRO-α在PCOS组的表达高于对照组,差异有统计学意义(P>0.05),两组IL-6表达差异无统计学意义(P>0.05)。结论炎性因子IL-8、TNF-α、IL-6和趋化因子GRO-α在PCOS的发生发展中可能起了重要的作用。PCOS可能是一种慢性炎性疾病。

Objective To quantify cytokine and chemokine mRNA expression in follicular fluid cells obtained at the time of oocyte retrieval before IVF in women with and without polycystic ovary syndrome （PCOS）. Methods Between January and August 2014, 80 subjects undergoing IVF-ET were chosen to be studied. Among them, 40 had PCOS, and the other 40 were controls. Follicular fluid cells were subjected to quantitative RT-PCR to evaluate growth-regulated oncogene-α （GRO-α）, tumor necrosis factor-α （TNF-α）, interleukins （IL-6, IL-8 ） expression relative to β-actin. And the chemokine was measured in serum and follicular fluids by immunoassays. These mRNA transcriptions production was proved by sequencing. Results GRO-α, TNF-α, IL-6 and IL-8 mRNA were expressed in follicular fluid cells. Their expressions were significantly higher in PCOS compared with controls （P〈 0.05）. They were also detected higher in PCOS than controls in serum and follicular （P 〈 0.05）, but the difference of IL-6 level between PCOS and controls in lean patients was not statistically significant （P 〉 0.05）. Conclusions GRO-α, IL-8, TNF-α, and IL-6 may play important roles in the occurrence and development of PCOS. PCOS may be a chronic immunological inflammatory disease.