蛋白水解诱导因子与胃癌患者营养状况及预后的关系

The relationship between proteolysis-inducing factor and the nutritional status and prognosis of gastric cancer patients

目的 检测蛋白水解诱导因子（PIF）在胃癌患者腹直肌及血清中的表达,探讨其表达与胃癌患者营养状况及预后的关系.方法 分别应用RT-PCR、Western blot和ELISA检测PIF在胃癌患者腹直肌和血清中的表达,分析其表达与胃癌患者营养状况及预后的关系.结果 PIF在胃癌组患者腹直肌组织中的表达较腹腔良性疾病组显著升高（P＜0.05）.在胃癌组中,60％ （32/53）腹直肌组织中PIF呈高表达,PIF表达的升高与患者体质量丢失、血清白蛋白及TNM分期显著相关（P＜0.05）.PIF在腹腔良性疾病组血清中表达显著低于胃癌组（P＜0.05）.PIF高表达胃癌患者的生存率低于PIF低表达者,差异有统计学意义（P＜0.05）.结论 PIF在胃癌患者腹直肌和血清中呈高表达,PIF表达水平与患者体质量减轻、血清白蛋白、TNM分期和预后密切相关,PIF可能在胃癌患者的恶病质过程中起重要作用.

Objective To investigate the prognostic significance of proteolysis-inducing factor （PIF） in gastric cancer patients.Methods Biopsies of the rectus abdominis muscle were obtained intra operatively from 53 gastric cancer patients and 53 subjects undergoing surgery for benign abdominal diseases,muscle and serum PIF,ubiquitin expression,and proteasome proteolytic activities were assessed through reverse-transcription polymerase chain reaction （RT-PCR）,Western blot,and enzyme-linked immunosorbent assay （ELISA）.Results PIF was significantly upregulated in muscle of gastric cancer compared to control muscles.PIF was upregulated in 60% （32/53） muscle of gastric cancer.Over expression of PIF in muscles of gastric cancer were associated with level of serum albumin,tumor node metastasis （TNM） stage,and percent of weight loss.The survival rate of patients with a high expression of PIF was significantly lower than those in patients with low expression （P 〈 0.05）.Conclusions PIF was significantly upregulated in muscle of gastric cancer,over expression of PIF in muscles of gastric cancer was associated with level of serum albumin,TNM stage,percent of weight loss,and prognosis.PIF might play an important role in progression and prognosis of gastric cancer.