IL-6和VEGF在慢性硬膜下血肿中的检测及意义

Detection of IL-6 and VEGF levels in chronic subdural hematomas and their meanings

目的探讨慢性硬膜下血肿(CSDH)患者白介素-6(IL-6)和血管内皮生长因子(VEGF)表达及意义。方法选取CSDH患者20例,均行钻孔引流术治疗,术中在骨孔内全层切取硬膜和血肿外膜,采用HE染色和免疫组化染色检查VEGF表达。选取同期健康体检者10例作为对照。采集CSDH患者血肿液和外周血以及对照组外周血,应用酶联免疫吸附法检测IL-6和VEGF浓度。结果 CSDH患者血肿外膜可见嗜酸性粒细胞、毛细血管增生及大量红细胞渗出;血肿外膜VEGF表达呈强阳性。CSDH血肿液和外周血IL-6和VEGF浓度均明显高于对照组(P<0.01),而且,CSDH患者血肿液IL-6和VEGF浓度明显高于其外周血浓度(P<0.01)。结论 CSDH患者血肿腔IL-6与VEGF含量增高,提示IL-6与VEGF可能相互促进,共同参与CSDH的形成。

Objective To explore the role of interleukin-6 （IL-6） and vascular endothelial growth factor （VEGF） in the development of chronic subdural hematomas （CSDH） and its mechanism. Methods The levels of IL-6 and VEGF in outer membrane of CSDH, hematomas fluid and the serum of peripheral venous blood were detected respectively by emzye-labelled immunosorbent assay and immunohestochemical technique in 20 patients with CSDH including 18 males and 2 females, who received the operation of burr hole and drainage （observed group） and the serum levels of IL-6 and VEGF in 10 healthy volunteers including 9 males and 1 female, who served as control group. Results （!）There were a number of Eosinophile granulocytes and red blood cells in CSDH and the many capillaries proliferated in the outer membrane of CSDH. The high expression of VEGF was observed in the outer membrance of CSDH. （2） There were insignificant difference in the serum levels of VEGF and IL-6 between both the groups （P〉0.05）. The levels of VEGF and IL-6 were significantly higher in CSDH fluid than those in the serum of the control group （P〈0.01）. Conclusions They are suggested that IL-6 may take part in the local inflammatory response of CSDH, VEGF may be an important factor in the formation of new vessels growth and IL-6 and VEGF may play an important role in the development of CSDH.