一次静脉注射纳米四氧化三铁对小鼠肝功能的影响

The effects of ferroferric oxide nanoparticles on liver function of mice after intravenous injection

目的研究纳米四氧化三铁（nano-Fe3O4）对小鼠肝功能的影响。方法 6~8周龄的CD-1（ICR）雄性小鼠81只,随机分为9组,分别将粒径为6.3和54.6 nm的nano-Fe3O4以10 mg/kg体重剂量进行尾静脉注射染毒;染毒后的1、3和7 d处死小鼠,测定小鼠肝脏系数、血清丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）水平;测定小鼠肝脏谷胱甘肽（GSH）水平,谷胱甘肽过氧化物酶（GSH-Px）与超氧化物歧化酶（SOD）活性,丙二醛（MDA）含量。结果与对照组比较,染毒后3和7 d,小鼠肝脏系数降低,差异有统计学意义（P〈0.05）;血清ALT、AST水平在染毒后1 d显著增高,差异有统计学意义（P〈0.05）,之后逐渐恢复正常水平;肝组织GSH水平在染毒后1 d,显著高于对照组（P〈0.05）;而染毒后1、3和7 d,小鼠肝脏GSH-Px和SOD活性、MDA含量,对照组与各组间差异均无统计学意义（P〉0.05）。结论单次尾静脉注射两种粒径的nano-Fe3O4,都能引起小鼠肝组织低水平的氧化应激,均未见肝组织明显损伤,两粒径的nanoFe3O4毒性无差异。

Objective To study the effects of ferroferric oxide nanoparticles（ nano-Fe3O4） on liver function of mice. Methods Eighty-one 6 ~ 8 weeks old healthy CD-1（ ICR） male mice were randomly divided into 9 groups; The mice were exposed to 6. 3 nm nanoFe3O4 groups and 54. 6 nm nano-Fe3O4 nanoparticle respectively by tail vein injection at a single dose of 10 mg / kg. The animals were sacrificed respectively on 1,3 and 7 days post-injection. The serum was collected for the analysis of glutamic-pyruvic transaminase（ ALT）and glutamic-oxalacetic transaminease（ AST）. The liver tissue was removed to determine the organ coefficient as well as the levels of glutathione（ GSH）,glutathione peroxidase（ GSH-Px）,superoxide dismutase（ SOD）,and malondialdehyde（ MDA）. Results On 3 and7 d,the organ coefficients of liver in both treatments were significantly lower than those in the control groups（ P〈0. 05）. The serum ALT and AST in both treatments significantly increased on day 1 post-injection（ P〈0. 05）,and returned to normal by 3 d and 7 d. No obvious effects on the GSH-px,SOD,MDA levels of liver tissue were found in both treatments（ P〉0. 05）,except for transient increases in the GSH on 1 d. Conclusion A single intravenous injection of nano-Fe3O4 with two different sizes can cause low-level oxidative stress in mice liver,but the exposure will not result in serious oxidative damages and chronic liver dysfunction. In addition,the two types of nano-Fe3O4 with different sizes did not show any different toxic effects.