摘要
The aim of the study was to investigate the anti-asthmatic effects of oxymatrine(OXY) and the possible underlying mechanisms.The mouse asthma model was established by ovalbumin(OVA) intraperitoneal injection.A total of fifty mice were randomly assigned to five groups:control,OVA,OVA + dexamethasone(Dex,2 mg·kg-1),and OVA + OXY(40 mg·kg-1),and OVA + OXY(80 mg·kg-1),respectively.Histological studies were conducted by the hematoxylin and eosin(HE) staining,the levels of interleukin-4(IL-4),interleukin-5(IL-5),interleukin-13,and Ig E were evaluated by enzyme-linked immunosorbent assay(ELISA),and the protein level of CD40 was analyzed by Western blotting.OXY inhibited OVA-induced increases in eosinophil count;the levels of IL-4,IL-5,Ig E,and IL-13 were recovered.It also substantially inhibited OVA-induced eosinophilia in lung tissues and the expression of CD40 protein.These findings suggest that OXY may effectively ameliorate the progression of asthma and could be explored as a possible therapy for patients with allergic asthma.
The aim of the study was to investigate the anti-asthmatic effects of oxymatrine （OXY） and the possible underlying mechanisms. The mouse asthma model was established by ovalbumin （OVA） intraperitoneal injection. A total of fifty mice were randomly assigned to five groups： control, OVA, OVA ＋ dexamethasone （Dex, 2 mgkg 1）, and OVA ＋ OXY （40 mg·kg^-1）, and OVA ＋ OXY （80 mg·kg^-1）, respectively. Histological studies were conducted by the hematoxylin and eosin （HE） staining, the levels of interleukin-4 （IL-4）, interleukin-5 （IL-5）, interleukin-13, and IgE were evaluated by enzyme-linked immunosorbent assay （ELISA）, and the protein level of CD40 was analyzed by Western blotting. OXY inhibited OVA-induced increases in eosinophil count; the levels of IL-4, IL-5, IgE, and IL-13 were recovered. It also substantially inhibited OVA-induced eosinophilia in lung tissues and the expression of CD40 protein. These findings suggest that OXY may effectively ameliorate the progression of asthma and could be explored as a possible therapy for patients with allergic asthma.
基金
supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions
