甜茶素对照品制备方法的研究

Preperation Method of Reference Substance of Rubusoside from Rubus suavissimus S.Lee

【目的】建立从甜茶叶中制备甜茶素对照品的方法,获得符合要求的甜茶素对照品。【方法】利用大孔树脂吸附、重结晶和液相色谱法对甜茶叶的水提取物——甜茶素进行分离、纯化结晶,然后采用薄层色谱法和高效液相色谱法对甜茶素进行纯度检查,并通过UV,IR,MS,NMR等对其进行结构鉴定。【结果】从甜茶叶水提取物中分离、纯化结晶出甜茶素对照品,质量分数W>99.0%。【结论】建立的制备方法简单可行,所得到的甜茶素对照品符合中药化学对照品的相关要求,可作为甜茶叶及其相关制剂的质量控制用化学对照品。

[Objective]A method for separating reference substance of Rubusoside from leave of Rubus suavissimus S.Lee was established in order to get the qualified reference substance of Rubusoside.[Methods]Water-extract of the leave of Rubus suavissimus S.Lee was isolated and purified by preparative liquid chromatography combining with macroporous resin adsorption and recrystallization.The purity of Rubusoside was checked by TLC and HPLC,and its struc-ture was identified by UV,IR,MS,NMR.[Results]Rubusoside was completely separated from the leave of Rubus suavissimus S.Lee,and the purity of the reference substance was no less than 99.0%.[Conclusion]The method is simple and convenient,by which the compound pre-pared is accorded with the relative demands of chemical reference substance in Chinese materia medica,and can be used as a reference subsatance for the quality control and the research of herbal medicine.