复方威茯急性毒性实验及抗炎镇痛作用观察

Study on Acute Toxicity,Anti-inflammatory and Analgesic Effects of Fufang Weifu

研究复方威茯的急性毒性作用,并观察其抗炎镇痛作用。以最大给药量方法进行急性毒性实验,观察小鼠一般情况等,14 d后处死解剖,观察小鼠主要脏器组织病变情况。以二甲苯致小鼠耳廓肿胀、醋酸致小鼠腹腔毛细血管通透性增加,实验观察复方威茯抗炎作用。以冰醋酸致小鼠扭体、热板致小鼠疼痛探讨复方威茯镇痛作用。结果在急性毒性实验中,未见小鼠有中毒症状或死亡,外观表现未见异常,解剖后未见脏器异常;复方威茯可明显抑制二甲苯所致小鼠耳廓肿胀度和醋酸致腹腔毛细血管通透性的增高(P<0.05或P<0.01),可明显减少醋酸致小鼠扭体反应次数(P<0.01),可提高热板致小鼠疼痛的痛阈值(P<0.05或P<0.01)。说明复方威茯无明显的急性毒性作用,有明显的抗炎镇痛作用。

To study the acute toxicity, anti-inflammatory and analgesic effects of Fufang Weifu, the acute toxicity experiment of Fufang Weifu was done through maximum dose of stomach perfusion in mice to observe their general state,etc. 14d later, mice was dissected to observe the lesions of major organs and tissues, ter dissected, heart, liver and other major organs and tissues lesions. Models of acute inflammatory reactions edema caused by xylene in mouse ear and acetic acid-induced abdominal capillary permeability increases to observe anti-inflammatory of compound Weifu. Methods of acetic acid-induced writhing and hot-plate test in mice to observe analgesic of compound Weifu. In the acute toxicological test, no clinical signs or death were found in mice, the general conditions,major organs and tissues of mice were not abnormal. Fufang Weifu can significantly counteracted xylene-induced mouse ear edema and acetic acid-induced elevation of abdominal capillary permeability（P 〈 0. 05 or P 〈 0. 01 ）. It could relieve the pain caused by acetic acid（P 〈0. 01） and hot-plate（P 〈0.05 or P 〈0. 01 ）. It is conclused that Fufang Weifu showed no significant acute toxicity, but markedly anti-inflammatory and analgesic effects in the study .