外周血和鼻咽组织EB病毒DNA检测对鼻咽癌诊断和防控的临床观察

Clinical significance of EB virus DNA testing in peripheral blood and nasopharyngeal tissues in diagnosis and prevention of nasopharyngeal carcinoma

目的探讨外周血和鼻咽组织EB病毒DNA检测对鼻咽癌诊断和防控的临床意义以及鼻咽腔冲洗对防控鼻咽癌的价值。方法选取2010年12月至2012年4月55例鼻咽癌患者为观察组,抽取同期55例健康者为对照组,采用EB病毒DNA PCR荧光定量检测技术检测外周血和鼻咽组织EB病毒DNA,比较鼻咽癌患者与健康者外周血和鼻咽组织EB病毒DNA检测结果。对另外1组67例EB病毒抗体阳性而鼻咽活检结果为阴性者进行鼻咽腔冲洗,比较治疗前、后EB病毒抗体检测结果。结果观察组患者外周血、鼻咽组织中EB病毒DNA阳性检出率均高于对照组健康者,差异均有统计学意义(均P<0.01)。经鼻咽腔冲洗治疗后,EB病毒抗体阳性者检测转阴率为19.4%,患者临床症状明显改善。结论鼻咽组织EB病毒DNA检测有助于鼻咽癌的临床诊断,对EB病毒抗体阳性者进行鼻咽腔冲洗治疗对于鼻咽癌的防控具有重要意义。

Objective To analysis the clinical significance of peripheral blood and nasopharyngeal tissues EB virus DNA testing on nasopharyngeal cancer diagnosis and prevention and the value of rinse the nasopharynx on prevention and control of nasopharyngeal. Methods 55 cases of NPC patients were ran- domly selected （observation group） in our hospital from December 2010 to April 2012, in the same period, 55 cases of healthy people were Extracted（ control group） , EB virus DNA was detected in peripheral blood and nasopharyngeal tissues with EB virus DNA PCR fluorescence quantitative detection technology, EB virus DNA test results in peripheral blood and nasopharyngeal tissues on NPC patients and healthy were com- pared. 67 cases of EB virus antibody-positive were treated with nasopharyngeal cavity rinse, EB virus anti- body test results before and after the treatment were compared. Results DNA positive rate in in peripheral blood and nasopharyngeal tissues of patients in the observation group was higher than that of healthy in con- trol group, the differences were statistically significant（P 〈 O. 01 ）. After treatment, EB virus testing negative rate of EB virus antibody-positive was 19.4%, the clinical symptoms on patients improved significantly. Conclusion It＇s of much use to detect EB virus DNA in nasopharyngeal tissues, nasopharyngeal cavity rinse has a significance in prevention of NPC.