红景天苷对链脲佐菌素所致糖尿病大鼠肝脏的保护作用

Protection of salidroside on hepatic in diabetic rats induced by streptozotocin

目的研究红景天苷对链脲佐菌素所致糖尿病大鼠肝脏的保护作用。方法一次性ip链脲佐菌素(60 mg/kg)制备糖尿病大鼠模型,取造模成功的糖尿病大鼠80只,按血糖水平随机分为模型组,红景天苷25、50、100 mg/kg组以及盐酸二甲双胍(25 mg/kg)组,每组16只;另取同龄正常饲养大鼠16只作为对照组。ip给药治疗,1次/d,疗程为12周。分别于0、4、8、12周测定各组大鼠血糖水平。治疗12周后,测定各组大鼠体质量和肝脏指数;检测血清中转氨酶(ALT、AST)、碱性磷酸酶(ALP)活性;测定肝脏组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量;并通过HE染色观察肝脏组织病理学改变。结果与模型组比较,红景天苷50、100 mg/kg组大鼠血糖水平显著降低(P<0.05、0.01),肝脏指数显著降低、体质量显著增高(P<0.05、0.01),血清中ALP活性明显降低(P<0.01),肝脏组织中SOD、CAT活性显著升高(P<0.05、0.01);红景天苷25、50、100 mg/kg组大鼠血清中转氨酶(ALT、AST)活性和肝组织中MDA含量显著降低(P<0.05、0.01);红景天苷组大鼠肝组织病理形态学改变程度明显减轻。结论红景天苷对链脲佐菌素所致糖尿病大鼠肝脏具有剂量相关性的保护作用,其作用机制可能与红景天苷能够有效改善抗氧化酶系统活性、降低氧化应激损伤,从而改善肝功能有关。

Objective To investigate protective effects of salidroside on hepatic in diabetic rats induced by streptozotocin （STZ）. Methods The experimental diabetic rat models were made by ip administered with STZ, and 80 models were selected. According to blood sugar levels, rats were randomly divided into the model group, salidros.ide （25, 50, and 100 mg/kg） groups, metformin hydrochloride （25 mg/kg） group, and other 16 rats as the control group. The drugs were given by ig administration for 12 weeks, once daily. In 0, 4, 8, and 12th weeks after experiment, the levels of fasting blood sugar were determined. After 12 weeks, the body weight and hepatic index were detected. The activities of ALT, AST, and ALP in serum were determined, and the activities of SOD and CAT, and the content of MDA in hepatic tissue were also determined. Histopathology changes of hepatic tissues were observed by HE staining. Results Compared with the model group, the fasting blood glucose level in salidroside 50 and 100 mg/kg groups significantly decreased （P 〈 0.01）, the hepatic index significantly decreased （P 〈 0.05, 0.01）, the body weight significantly increased （P 〈 0.05, 0.01）, the activity of ALP significantly decreased （P 〈 0.01）, and the activity of SOD and CAT in the hepatic tissue significantly increased （P 〈 0.05, 0.01）. The activities of ALT and AST in salidroside 25, 50, and 100 mg/kg groups were significantly decreased （P 〈 0.05, 0.01）, and the content of MDA in the hepatic tissue also significantly decreased （P 〈 0.05, 0.01）. The histopathology changes of hepatic tissue in salidroside groups were significantly improved. Conclusion Salidroside has the protective effect on hepatic in diabetic rats induced by STZ with dose-dependent relation, whose mechanism may be related to its effects of improving antioxidant ability, reducing the damage of free radical, and improving hepatic function.