玉米内生细菌Y19的荧光标记及其在玉米体内的定殖应用效果

GFP-tagging and Colonization Effect of an Endophityic Bacterial Strain Y19 in Maize

Y19是从玉米种子分离的具有促生、防病效果的解淀粉芽孢杆菌。为探究其在玉米体内及根围土壤中的定殖情况,通过自然转化方式将含有绿色荧光蛋白基因gfpmut3a的质粒p HAPII导入Y19,获得具有良好荧光表型的标记菌株Y19-GFPmut3a。室内平板对峙、玉米盆栽促生定殖实验的结果表明,质粒的引入对Y19的拮抗病原及促生效果并无明显的影响,接种后的第40天,仍能在玉米的根围土壤、根、茎及叶组织中检测到标记菌株;激光共聚焦显微镜观察结果显示,接菌后的第9天,即可观察到其在玉米根、茎及叶部组织的定殖,表明Y19在玉米体内具有良好的运输传导性能。

Bacillus amyloliquefaciens strain Y19 isolated from maize seed has the capacity to promote plant growth and control disease. To investigate its colonization into maize tissues and rhizosphere, a tagged strain Y19- GFPmut3a with good fluorescence was obtained through transformation of pHAPII into Y 19 by natural competence. The introduction of exogenous plasmid has no significant effect on the antagonism and plant growth-promotion of Y19, according to the plate antagonistic bioassay and maize pot experiment in the greenhouse. In 40 days after inoc- ulation （DAI）, the gfp-tagged strain still could be detected in rhizospheric soil, roots, stems and leaves tissues of maize, meanwhile its colonization into roots, stems and leaves was observed at 9 DAI by confocal laser scanning mi- croscope. These indicated that Y19 owns a good transduction in host plant.