不同肥力土壤中分解几丁质微生物代谢产物对玉米的促生效果研究

Effect of Decomposition Chitin Microbial Metabolites on Maize Growth-promoting under Different Fertility Soils

从吉林省不同玉米栽培区土壤中以胶状几丁质为碳源分离筛选分解几丁质微生物,最终筛选出功能菌株并命名为CC-1,采用16 Sr RNA序列扩增法鉴定为芽孢杆菌Bacillus sp,通过SDS-PAGE分析法进一步确定该菌株的几丁质酶生产能力和几丁质酶的蛋白分子量为30 k Da。通过盆栽试验进行微生物肥料对不同肥力中的玉米促生效果的研究,结果表明,以CC-1菌株生产的微生物肥料效果受土壤的基础肥力状况影响较大,土壤的肥力状况与微生物的增产效应成反比,相对贫瘠的土壤对微生物处理的响应较为敏感,对玉米的生物学性状及产量表现出了一定的促进作用,增产达到了13.08%。

One strain of Chitin microbial bacterium was isolated from the different maize cultivation areas in Ji- lin province, with colloidal chitin as their carbon source, and named CC-1, and finally identified as Bacillus sp by 16 SrRNA sequence amplification method. In addition, using SDS-PAGE analysis to further confirm this strains chi- tinase production capcity and chitinase molecular weight of proteins. And by the pot experiment on different fertility soil to study the effect of decomposition chitin microbial metabolites of maize growth. The experiments demonstrated that there was a positive effect on biological character and yield, but the effects of microbial fertilizer was greatly in- fluenced by soil basis fertility conditions. The results showed that the fertility of the soil condition was inversely pro- portional to the microbial stimulation effect. By comparison, poor soil had more sensitive to microbial treatment than fertile soil, showed a certain role on the biological characteristics and yield of maize, yield reached 13.08%.