鸡源奇异变形杆菌的分离鉴定及耐药性研究

Isolation and identification of Proteus mirabilis from chicken and study of its drug resistance

为了考察奇异变形杆菌的耐药情况,试验定点采集养殖场肉鸡泄殖腔棉拭子1 829份,经分离、纯化、鉴定获得奇异变形杆菌67株,分离率为3.66%;并用11种抗生素对67株奇异变形杆菌进行药敏试验。结果表明:该地区鸡源奇异变形杆菌对11种抗生素的耐药性普遍偏高,平均耐药率达到80.00%以上的药物有黏杆菌素E、氟苯尼考、磺胺异唑、恩诺沙星、复方新诺明、氨苄西林、多西环素、大观霉素和四环素9种药物,对庆大霉素的耐药率为62.69%,对头孢噻呋耐药率为0。说明奇异变形杆菌在肉鸡养殖场具有较高的分离率,是兽医临床需要重点监控的致病菌之一。

To investigate the drug resistance of Proteus mirabilis, 1 829 cotton swabs of cloacal samples were collected from the broilers in the appointed poultry farms. 67 strains of Proteus mirabilis were obtained through isolation, purification, and identification, and the isolation rate was 3.66%. The 67 strains of Proteus mirabilis were tested for drug resistance using eleven kinds of antibiotics. The results showed that the Proteus mirabilis from the region generally possessed higher levels of drug resistance against the eleven kinds of antibiotics. The average resist- ance rates reached more than 80.00% in the nine kinds of drugs including colistin E, florfenicol, bacteresulf, enrofloxacin, cotrimoxazole, am- picillin, doxycycline, spectinomycin and tetracycline. The drug resistance against gentamicin was 62.69% and the drug resistance against ceft- iofur was zero. The results indicate that Proteus mirabilis has a higher isolation rate in the broiler farms, and is one of the pathogens that need to be focused on monitoring in the clinic by veterinarian.