结直肠癌患者幽门螺杆菌感染与高胃泌素血症及环氧合酶-2的相关性研究

Hp infection in colorectal cancer and its relationship with hypergastrinemia and cyclooxygenase-2

目的观察结直肠癌患者幽门螺杆菌(Hp)感染情况,探讨Hp与高胃泌素血症及环氧合酶-2(COX-2)的相关性,为制定控制感染对策提供依据。方法选择2009年12月-2013年12月在医院治疗的结直肠癌患者121例,另选择同期来医院进行经结肠镜检查,并且未发现明确的消化道疾病患者98例临床资料,采用酶联免疫吸附法检测其抗Hp IgG水平,并采用放射免疫分析法和免疫组织化学染色法检测胃泌素和COX-2水平变化,数据采用统计软件SPSS17.0进行分析。结果结直肠癌患者Hp阳性率、高胃泌素血症发生率及COX-2阳性率分别为70.2%、52.9%和62.8%,均明显高于对照组的12.2%、15.3%和9.2%,差异有统计学意义(P<0.05);结直肠癌患者Hp阳性与肿瘤发生部位、TNM分期和淋巴结转移明显相关,差异有统计学意义(P<0.05);结直肠癌Hp阳性患者高胃泌素血症发生率及COX-2阳性率分别为64.7%、89.4%,均明显高于Hp阴性患者的25.0%、38.9%,两组比较差异均有统计学意义(P<0.05)。结论结直肠癌患者Hp阳性率明显升高,并且与高胃泌素血症发生及COX-2表达相关,为揭示结直肠癌的病因和制定新的治疗策略提供了理论依据和思路。

OBJECTIVE To observe the infection of Helicobacter pylori （Hp） in colorectal cancer and discuss its relationship with hypergastrinemia and cyclooxygenase‐2 （COX‐2） ,so as to provide the basis for the development of countermeasures for controlling infections .METHODS Totally 121 patients with colorectal cancer treated in the hospital during Dec .2009 to Dec .2013 were enrolled and 98 patients who were not confirmed with digestive diseases by colonoscopy during the same period were also enrolled .The levels of anti‐Hp IgG were detected in all the patients using enzyme‐linked immunosorbent assay .The levels of gastrin and COX‐2 were detected by radioim‐munoassay and immunohistochemical staining . The statistical software SPSS17 .0 was used for data analysis . RESULTS The positive rate of Hp infection ,the incidence of hypergastrinemia and the positive rate of COX‐2 were 70 .2% ,52 .9% and 62 .8% in colorectal cancer patients ,which was significantly higher than 12 .2% ,15 .3% and 9 .2% in the control group （P〈0 .05） .The Hp infection in colorectal cancer patients was significantly correlated with tumor location ,TNM stage and lymph node metastasis（P〈0 .05） .The incidence of hypergastrinemia and the positive rate of COX‐2 in Hp‐positive colorectal cancer patients were 64 .7% ,89 .4% ,which was significantly higher than 25 .0% ,38 .9% in Hp‐negative colorectal cancer patients （P〈0 .05） .CONCLUSION The positive rate of Hp is significantly increased in colorectal cancer patients ,and correlated with hypergastrinemia and COX‐2 ,providing theoretical basis and ideas for revealing the etiology of colorectal cancer and the development of new therapeutic strategies .