支气管哮喘患者血浆中IL-33及可溶性ST2的水平和临床意义

Changes of plasma interleukin 33 and its soluble receptor ST-2 in asthmatic patients and its clinical significance

目的通过检测支气管哮喘（简称哮喘）患者血浆中自介素33（IL-33）和可溶性ST-2（sST-2）的表达水平，探讨哮喘患者血浆中IL-33和sST-2的作用和临床意义。方法①用ELISA法检测60例哮喘急性发作期患者和20例健康体检者外周血中IL-33和sST-2的血浆浓度。②对所有哮喘患者行肺功能检测（PTF）。③依据FEV1将哮喘患者分为3组：FEV1≥80％或PEF≥80％为轻度哮喘组，60％≤FEV1〈80％或60％≤PEF〈80％中度哮喘组，FEV1〈60％或PEF〈60％为重度哮喘组。结果①轻、中、重度哮喘急性发作期患者血浆中IL-33的水平较正常对照组升高（F=454．81，P=0．00），差异有统计学意义（P〈0．05）。②轻、中、重度哮喘急性发作期患者血浆中sST-2水平较正常对照组升高（F=96．71，P=0．00），差异有统计学意义（P〈O．05）。③哮喘急性发作期患者血浆中IL-33水平与FEV1％pred和PEF均呈明显负相关（P〈0．05）。结论IL-33和sST-2均参与了哮喘的发病过程，其血浆水平的变化可做为病情监测的一项血清学指标，且IL-33可作为重症哮喘和肺功能严重受损的一个标识。

Objective To study the changes of plasma interleukin-33 （IL-33） and its soluble receptor ST-2 （sST-2） in asthmatic patients,and investigate the clinical significance and the effect of IL-33 and sST-2 signal pathway in bronchial asthma （asthma）. Methods The plasma levels of IL-33 and its sST-2 were detected by ELISA method in 60 patients with asthma,who were divided into the acute attack group,and 20 healthy controls. Of all patients with asthma pulmonary function test （PTF）. ③ According to the pulmonary function （FEV1%pred） in patients with asthma were divided into 3 groups： FEV1≥ 80% and PEF≥80% for mild asthma group, 60≤FEV1〈80%, 60% 〈GPEF〈80% moderate asthma group,FEV1〈60% or PEF〈60% for patients with asthma. Results ①The plasma level of IL-33 with acute exacerbation of asthma in the mild group and moderate group and severe group was markedly increased compared with normal control group （ F = 454.81, P = 0.00）, the differences were statistically significant （ P〈0.05）. ②The plasma level of sST-2 in acute asthma with acute exacerbation of asthma in the mild group and moderate group and severe group was markedly increased compared with normal control group （ F = 96.71, P = 0.00）, the differences were statistically significant （ P 〈0.05）. ③The level of IL-33 were negatively correlated with FEV1% pred and PEF of acute exacerbation of asthma （ P 〈0.05）. Conclusions IL-33 and its receptor sST-2 were involved in the pathogenesis of asthma,changes to the plasma levels of a serological index for monitoring disease progression,and IL-33 can be used as a sign of severe asthma and lung function is damaged badly.