棘豆扶正胶囊中苦马豆素的检测

Detection of swainsonine in whin capsules by HPLC-MS

目的：采用液相色谱串联质谱法（HPLC—MS）检测棘豆扶正胶囊中的苦马豆素。方法：取一定量胶囊内容物加1％甲酸甲醇溶液提取后，净化过滤，上机测定。结果：在0．5～50．0μg·L^-1浓度范围内线性良好，相关系数r：0．9994，最低定量限为0．1μg·L^-1，RSD为2．6％，加标回收率92．4％～96．6％。结论：该方法前处理简便快捷，能够避免植物提取物中基质效应的影响，精密度、准确度良好，检测结果准确可靠。

Objective： Detect swainsonine in whin capsules by HPLC-MS. Methods： The sample was soaked with 1% formic acid methanol then purified and detected. Results ：The correlation coefficient of this method in 0.5 - 50.0 μg.L^-1 concentration is 0. 999 4. The minimum quantitative limit is 0.1 μg.L^-1. The RSD is 2. 6%. The recovery is 92.4% - 96.6%. Conclusion： This method is simple in sample preparation and has good precision, accuracy and stability.