无机多孔铈基材料的生物相容性研究（英文）

Biocompatibility Study of Inorganic Porous Cerium-based Materials

采用晶胶模板法在载玻片上制备了无机多孔铈基材料,并对其物相结构和形貌进行了表征.以人体胃癌细胞(BGC-803)为模型细胞,评价了无机多孔铈基材料对BGC-803细胞的生物相容性.将BGC-803细胞在浸有无机多孔铈基材料载玻片的培养液中进行培养,分不同时间段对BGC-803细胞的在无机多孔铈基材料载玻片上成活率进行测试,研究结果表明BGC-803细胞在无机多孔铈基材料上具有较好的黏附特性,无机多孔铈基材料对BGC-803细胞具有较低的毒性和较好的生物相容性.细胞迁移研究结果表明,无机多孔铈基材料的孔结构虽然可提高细胞黏附特性,但会引起小范围内细胞集落化,显示出在组织再生工程领域有潜在的应用价值.本文有助于我们深入了解无机多孔铈基材料的生物相容性,以便利用其作为生物支架材料应用于生物医学组织工程领域提供依据.

Inorganic porous materials have attracted considerable research interests due to their wide applications in biocatalysis,biosensing,and bioseparation.In this paper,inorganic porous cerium-based materials were synthesized and insitu deposited on microscope slides via a crystal colloidal templating method and their structure,size,and morphology were analyzed.Furthermore,the biocompatibility of inorganic porous cerium-based materials as cell culture scaffolds was evaluated using human gastric cancer cells（BGC-803）as model system.By comparing the cell viability and migration of BGC-803 cells cultured on the dish,the blank slide,and the inorganic porous cerium-based material deposited slide,it is revealed that inorganic porous cerium-based materials show fine adhesive ability,low cytotoxicity,and relatively good biocompatibility for BGC-803 cells.In comparison with the cells cultured on the dish and the blank slide,the cells cultured on inorganic porous cerium-based material deposited slide show migration property and cell colonization in a small area,which may be due to their porous structure and inorganic skeleton.The study may help us further evaluate the feasibility of using inorganic porous cerium-based materials as scaffolds/supports for modulating the cellular adhesion,proliferation,and migration.Our results also indicated that the materials are biocompatible and suitable for potential tissue regeneration applications,such as the reconstruction of bones.