超临界CO_2萃取长裙竹荪总黄酮的工艺优化及抗氧化研究

Study on optimization of extraction of total flavonoids from Dictyophora indusiata Fisch. by supercritical CO_2 and its antioxidant activity

通过单因素实验探讨了萃取温度、CO2流量、萃取压力、萃取时间、夹带剂用量、分离试剂种类及用量对长裙竹荪总黄酮得率的影响,并采用响应面分析法确定超临界CO2萃取长裙竹荪总黄酮的最佳工艺条件为:萃取温度40℃,CO2流量10L/h,萃取压力35.5MPa,萃取时间122min。在此条件下,添加2∶1(V∶m)的95%乙醇作为夹带剂,以1∶4(V∶V)的石油醚对粗提物进行分离纯化,得到的长裙竹荪总黄酮得率可达3.6mg/g。抗氧化实验表明,长裙竹荪提取物有很好的还原性,对超氧阴离子(O2-·)、DPPH·和羟基自由基都有较好的清除能力,具有良好的抗氧化性。

The effect of extraction temperature,CO2 flow rate,extraction pressure,extraction time,entrainer volumes, types and amounts of separation solvents on the extraction yield of total flavonoids from Dictyophora indusiata Fisch. was investigated by single factor test in this study. The supercritical CO2 extraction conditions were further optimized by the response surface analysis and were determined to be extraction temperature of 40℃, CO2 flow rate of 10L/h,extraction pressure of 35.5MPa and extraction time of 122min. Under the optimal extraction conditions,the yield of total flavonoids from Dictyophora indusiata Fisch. reached 3.6mg/g by adding 2：1 （V：m）95% ethanol and then adding 1：4（V：V） petroleum ether. Result of antioxidation showed that the total flavonoids from Dictyophora indusiata Fisch. had good total reduction capabilities,and scavenging hydroxyl radicals, DPPH, superoxide anion（Q2^-. ）, it had good antioxidant activity.