大孔吸附树脂分离纯化苦荞总皂苷的研究

Study on separation and purification of total saponins from tartary buckwheat with macroporous resin

为优化大孔吸附树脂分离纯化苦荞总皂苷的工艺条件,通过静态吸附解吸实验筛选出适合分离纯化苦荞总皂苷的大孔吸附树脂SP700,其饱和吸附量为（25.241±0.590）mg皂苷/g树脂。研究了样液浓度、吸附时间对吸附容量的影响,乙醇体积分数对解吸得率的影响,并进行了动态实验,确定了SP700型大孔树脂分离纯化苦荞总皂苷的最佳工艺条件为：最佳上样浓度约0.586mg/m L,流速2BV/h,树脂比样液体积为1∶1,动态洗脱实验中,上样后用体积分数分别为50%和70%的乙醇溶液进行分段洗脱,洗脱流速为2BV/h,用量为2~3BV,洗脱得率最高可达到88.9%,洗脱液蒸干后所得固形物中皂苷含量较提取液固形物中皂苷含量提高了约2倍。

In order to optimize process condition of separation and purification of total saponins from tartary buckwheat with macroporous resin. The appropriate SP700 resin was choosed,which the saturated adsorption capacity was up to （25.241±0.590）mg saponins/g resin through the static adsorption and desorption test. The influence of sample liquid concentration,adsorption time on the adsorption capacity,and the effect of ethano} volume fraction on the yield of desorption were studied in the static test. And in the dynamic test,the optimal purification conditions of using SP700 resin to absorb and purify total saponins from tartary buckwheat were as follows ： the best concentration of sample solution 0.586mg/mL,velocity 2BV/h,the ratio of resin and sample solution 1：1 ,in the dynamic elution experiment,segmented elution was carried with 50% and 70% ethanol solution respectively elution velocity 2BV/h,dosage 2~3BV,elution rate was up to 88.9%,the content of saponins in eluent was 2 fold as that in sample solution.