响应面法优化盐藻β-胡萝卜素超声波提取工艺

Optimization of extracting technique assisted by ultrasound assisted extraction of β-carotene from Dunaliella salina by response surface methodology

对盐藻β-胡萝卜素超声波辅助提取工艺参数进行了优化研究。结果表明,在所选择的几种溶剂中,无水乙醇的提取效果最好,以β-胡萝卜素得率为衡量指标,各溶剂提取效率的顺序为:无水乙醇>95%乙醇>无水乙醇和乙酸乙酯混合溶液(2∶1,v/v)>石油醚>三氯甲烷>乙腈>乙酸乙酯。通过响应面实验对超声波提取盐藻β-胡萝卜素的工艺进行了优化,得到了相应的数学模型,在所选的各因素水平范围内,各因素相互间均没有交互作用,三个考查因素对盐藻中β-胡萝卜素得率影响的顺序为:超声波功率>超声时间>液固比,确定超声波辅助提取β-胡萝卜素的最佳工艺参数为:液固比500m L/g、超声波功率496W、超声时间7min,β-胡萝卜素得率的预测值为1.282%,在优化条件下,β-胡萝卜素得率测定值为1.273%,说明提取模型的适应性良好。

Extracting technique assisted by ultrasound assisted extraction method of β-carotene from D. Salina was optimized. The result showed that absolute ethanol was the optimal solvent among these kinds of solvent selected,the β-carotene yield of D. Salina was used as measurement index,the sequence of extraction efficiency of different solvent was that absolute ethanol〉95% ethanol〉absolute ethanol/acetic ether（2：1,v/v）〉petroleum〉 chloroform〉acetonitrile〉acetic ether. The mathematical model of ultrasound assisted D. Salina β-carotene extraction was established by response surface methodology,within the scope of each factor levels,there was no interaction between different factors. The sequence of the effect of three factors on β-carotene yield was that ultrasonic power〉ultrasonic time〉ratio of liquid to solid. The optimal condition for extraction technique was that L/S 500mL/g, ultrasonic power 496W, ultrasonic time 7min, under the optimal condition,the predicted β-carotene yield was 1.282%,and the measured value was 1.273%,it was described that the adaptable of model was good.