骨关节炎患者血清及关节液中白细胞介素-17表达及其意义

Expression of interleukin-17 and its biological significance in serum and synovial fluid of knee osteoarthritis

目的：检测膝骨关节炎（osteoarthritis，OA）患者血清及关节液中白细胞介素-17（in-terleukin-17）的表达并评估其与 OA 严重程度的相关性。方法收集100例原发性膝 OA 患者血清及关节液标本，并以50例健康志愿者血清标本作为对照。OA 严重程度及分级分别采用 Lequesne指数和 K-L 分级系统。酶联免疫吸附法分别检测血清及关节液中 IL-17浓度，并分析其与 OA 严重程度的相关性。结果OA 组患者血清中 IL-17浓度[（5．292±1．470）pg／ml]明显高于对照组[（4．173±0．640）pg／ml]，差异有统计学意义（P ＜0．05）。K-L4级或3级 OA 患者关节液 IL-17的浓度明显高于2级 OA 患者，差异有统计学意义（P ＜0．05）。4级 OA 患者关节液 IL-17的浓度[（6．161±1．120）pg／ml]明显高于3级 OA 患者[（4．474±0．816）pg／ml]，差异有统计学意义（P ＜0．05）。关节液 IL-17浓度与 Lequesne 指数呈正相关（r ＝0．6232，P ＜0．05）；血清 IL-17浓度与 Lequesne 指数之间无明显相关。结论IL-17在 OA 患者血清及关节液中的表达明显升高，关节液中 IL-17的水平与 OA 严重程度之间明显相关，提示 IL-17的失衡表达在 OA 的发病机制中具有一定作用。

Objective To determine the interleukin（IL）-17 concentrations in serum and synovi-al fluid from patients with knee osteoarthritis（OA）,and evaluate their correlation with disease severity. Methods Serum and synovial fluid were collected from 100 patients with primary knee OA;age-and sex-matched 50 healthy subjects provided serum samples as controls.OA severity and grade were assessed u-sing the Lequesne index and Kellgren and Lawrence（K-L）grading system,respectively.The expression of IL-17 was quantified by enzyme-linked immunosorbent assay.Results Serum IL-17 concentrations were significantly higher in patients than in controls（P 〈0.05）.In the patient group,patients with K-L grade 3 and 4 had higher synovial IL-17 levels than those with K-L grade 2（P 〈0.05）.At the same time,com-pared with K-L grade 3,there was a higher IL-17 level in K-L grade 4 patients（P 〈0.05）.Synovial fluid IL-17 concentration was positively correlated with Lequesne index（r =0.6232,P 〈0.05）.There was no correlation between serum IL-17 concentrations and Lequesne index,however.Conclusion The expres-sion of IL-17 was significantly increased in serum and synovial fluid of knee from patients with knee OA. There was a positive correlation between synovial fluid IL-17 concentrations and OA severity.It suggests that the imbalance of IL-17 expression plays a certain role in the pathogenesis of OA.